Development and validation of a method for quantitative determination of the amount of fructosans in chicory roots
- Авторлар: Gulenkov А.S.1, Kulyak O.Y.1,2, Dul V.N.1, Mavrina P.O.1, Zotov I.E.3, Tomilina A.Y.3, Mizina P.G.1
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Мекемелер:
- All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
- Lomonosov Moscow State University
- D. Mendeleev University of Chemical Technology of Russia
- Шығарылым: Том 28, № 8 (2025)
- Беттер: 27-37
- Бөлім: Pharmaceutical chemistry
- URL: https://journals.rcsi.science/1560-9596/article/view/310626
- DOI: https://doi.org/10.29296/25877313-2025-08-04
- ID: 310626
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
Introduction. To develop regulatory documentation for raw materials of chicory roots, it is necessary to standardize the raw materials according to the content of the main active substance, which is fructosans (the main substance is inulin) [1, 2]. The Russian Pharmacopoeia 15th edition, includes articles ФС.2.5.0070.18, ФС.2.5.0025.15, which include a method for determining the amount of fructosans in a plant, based on the spectrophotometric determination of the absorption of a colored complex of acid hydrolysis products of fructosans - 5-hydroxymethylfurfural (5-HMF) with resorcinol. However, in the scientific literature there are methods based on determining the absorption of hydrolysis products without additional complex formation.
The aim – development and validation of a method for the quantitative determination of the amount of fructosans in terms of inulin in chicory roots with spectrophotometric determination of 5-HMF absorption, without the use of additional reagents (resorcinol, thiourea).
Material and methods. The object of the study is the roots of common chicory. The development and validation of the method for the quantitative determination of the sum of fructosans in terms of inulin was carried out by spectrophotometry.
Results. The developed method has been positively evaluated in the following parameters: specificity (coincidence of absorption maxima at a wavelength of (286±2) nm); linearity (correlation coefficient 0.9987); accuracy (range of recovery percentage – from 96.59% to 103.50% and has an average value of 99.97%); repeatability (coefficient of variation (RSD) 1.52% < 2.00%); reproducibility (coefficients of variation of intralaboratory reproducibility do not exceed 2%).
Conclusion. The obtained results allow us to consider the developed method suitable for reliable quantitative determination of the amount of fructosans in terms of inulin in the roots of common chicory.
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##article.viewOnOriginalSite##Авторлар туралы
А. Gulenkov
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Хат алмасуға жауапты Автор.
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0002-1532-9330
SPIN-код: 9436-3210
Ph.D. (Pharm), Leading Researcher, Department of Chemistry of Natural Compounds
Ресей, 7 Grin str., Moscow, 117216O. Kulyak
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants; Lomonosov Moscow State University
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0003-0385-3474
SPIN-код: 9697-1220
Ph.D. (Pharm), Leading Researcher, Department of Chemistry of Natural Compounds, Head of the Testing Center, Associate Professor of the Department of Pharmaceutical Chemistry and Organization of Pharmaceutical Affairs of the Medical Scientific and Educational Institute
Ресей, 7 Grin str., Moscow, 117216; Leninskie Gory 1, Moscow, 119991V. Dul
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0001-7824-4417
SPIN-код: 8192-5803
Ph.D. (Pharm.), Leading Researcher, Department of Chemistry of Natural Compounds
Ресей, 7 Grin str., Moscow, 117216P. Mavrina
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0002-8559-8771
SPIN-код: 7119-8667
Post-graduate Student, Junior Researcher, Department of Chemistry of Natural Compounds
Ресей, 7 Grin str., Moscow, 117216I. Zotov
D. Mendeleev University of Chemical Technology of Russia
Email: gulenkov@vilarnii.ru
ORCID iD: 0009-0005-0705-8672
Student of the Faculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, Department of Chemistry and Technology of Biomedical Drugs
Ресей, Miusskaya square 9, Moscow, 125047A. Tomilina
D. Mendeleev University of Chemical Technology of Russia
Email: gulenkov@vilarnii.ru
ORCID iD: 0009-0009-7751-4310
Student of the Faculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, Department of Chemistry and Technology of Biomedical Drugs
Ресей, Miusskaya square 9, Moscow, 125047P. Mizina
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0001-6510-9603
SPIN-код: 7084-1962
Dr.Sc. (Pharm.), Professor, Advisor to the Director, Honored Scientist of the Russian Federation
Ресей, 7 Grin str., Moscow, 117216Әдебиет тізімі
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- Kopytko Ya.F. Determination of total carbohydrates (recalculated for fructose upon conversion to furans) in burdock juice. Khimiko-Farmatsevticheskii Zhurnal. 2017; 51(4): 38–40. (In Russian).
- Bokov D.O. Hromchenkova E.P., Sokurenko M.S. et.al. Development of a technique for the determination of inulin in natural instant chicory after enzymatic hydrolysis by high-performance liquid chromatography. Voprosy pitaniia [Problems of Nutrition]. 2017; 86(5): 50–55. (In Russian).
- Dyakova N.A. Development and validation of the express technique of allocation and quantitative definition of water-soluble polysaccharides of leaves of Arctium Lappa L. Khimiya Rastitel’nogo Syr’ya [Chemistry of plant raw material]. 2018; 4: 81–87. (In Russian).
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