Comparative study of extraction methods from biological material of tricyclic antidepressants: imipramine, clomipramine, amitriptyline
- Авторлар: Kulikova V.K.1, Kirichek A.V.1,2, Shabalina A.E.2, Raspopova N.А.1, Podde S.A.1
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Мекемелер:
- D.I. Mendeleev Russian University of Chemical Technology
- 111 Main State Center for Forensic and Criminalistic Examinations of the Ministry of Defense of the Russian Federation
- Шығарылым: Том 28, № 4 (2025)
- Беттер: 23-31
- Бөлім: Pharmaceutical chemistry
- URL: https://journals.rcsi.science/1560-9596/article/view/290925
- DOI: https://doi.org/10.29296/25877313-2025-04-03
- ID: 290925
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Аннотация
Introduction. Tricyclic antidepressants (TCAs) are historically the first group of medications used to treat depression. They work by affecting the duration of monoamines presents in the presynaptic cleft in the brain and blocking certain receptors, such as m-cholinergic and alpha-adrenergic. TCAs can cause a toxicological effect and lead to fatal poisoning. This group of drugs is often used among drug addicts, as well as in the criminal environment.
The purpose of the study. Determination of an optimal and effective method for TCAs extraction from biological material using solid-phase (SPE) and liquid-liquid extraction (LLE) for further chemical-toxicological studies.
Materials and methods. The objects of the study were representatives of the TCAs group: imipramine, amitriptyline and clomipramine. Urine was used as a biological material. In the study of SPE method columns produced by Agilent Technologies company were used. These columns contained Evidex and ODS-C18 sorbents. Analutes isolation using LLE were realised according to the one of the most common methods. Analisys were conducted on gas-chromatography with a mass-selective detector (GC/MS).
Results. The method of extraction on a solid sorbent was developed using phosphate buffer with pH 4.8. After using the LLE method, samples with high contamination of co-extracted impurities of biological origin were observed. The error in the results of TCAs extraction using SPE was less than 10%, while the error for the LLE method was 25-35%. A comparison of two different extraction methods was studied using various TCAs concentrations. The advantages of using the ODS-C18 as a sorbent for SPE were determined. Calibration curves were constructed for samples using the SPE method. Limits of detection and quantification for all TCAs were determined.
Conclusions. The advantages of isolating TCAs using the SPE method over the LLE method were revealed.
Толық мәтін
##article.viewOnOriginalSite##Авторлар туралы
V. Kulikova
D.I. Mendeleev Russian University of Chemical Technology
Хат алмасуға жауапты Автор.
Email: v.k.kulikova@gmail.com
ORCID iD: 0009-0002-7592-9108
SPIN-код: 7158-6140
Student
Ресей, Miusskaya square, 9, building 1, Moscow, 125047A. Kirichek
D.I. Mendeleev Russian University of Chemical Technology; 111 Main State Center for Forensic and Criminalistic Examinations of the Ministry of Defense of the Russian Federation
Email: AVK_SUD@mail.ru
ORCID iD: 0009-0006-2202-3168
SPIN-код: 2844-2985
Senior Lecturer, Department of Expertise in Doping and Drug Control, Forensic Medical Expert, Department of Forensic Chemical Expertise
Ресей, Miusskaya square, 9, building 1, Moscow, 125047; Gospitalnaya Ploshchad, d. 3, building 16, Moscow, 105094A. Shabalina
111 Main State Center for Forensic and Criminalistic Examinations of the Ministry of Defense of the Russian Federation
Email: angelisha81@yandex.ru
ORCID iD: 0009-0001-0383-9464
Ph.D. (Chem.), Head of the Forensic Chemical Examination Department
Ресей, Gospitalnaya Ploshchad, d. 3, building 16, Moscow, 105094N. Raspopova
D.I. Mendeleev Russian University of Chemical Technology
Email: raspopova_nadezhda@inbox.ru
ORCID iD: 0009-0007-6265-1027
Student
Ресей, Miusskaya square, 9, building 1, Moscow, 125047S. Podde
D.I. Mendeleev Russian University of Chemical Technology
Email: comingoutlarry@gmail.com
ORCID iD: 0009-0007-4015-7343
Student
Ресей, Miusskaya square, 9, building 1, Moscow, 125047Әдебиет тізімі
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