POLYMORPHISM OF EPSTEIN-BARR VIRUS LMP1 ONCOGENE IN NANAIANS, REPRESENTATIVES OF INDIGENOUS MINORITY OF THE RUSSIAN FAR EAST


Cite item

Full Text

Abstract

The mechanism of EBV-associated malignant and benign human pathologies in non-endemic regions is still not elucidated. The investigation of this problem in Russia, the country, non-endemic for EBV-associated diseases, is of a special importance due to the variety of ethnic groups inhabiting different geographic and climatic regions. The search for genetic peculiaritis of EBV strains persisting in indigenous peoples of Russia, especially, in its minority representatives occupying the country since historical times is of the particular interest. Epstein-Barr virus (EBV) is known to be associated with a number of human tumors of lymphoid and epithelial cell origin. This unique feature of EBV is polymorphism of its main oncogene - latent membrane protein 1 (LMP1), encoded by a gene of the same name LMP1. The importance of the studying of genetic mutations (deletions, insertions and other) in this gene is based on the influence of his certain mutations on the activity of such key intracellular molecules as NF-kB, AP-1, iNOS, and several others, leading to cell malignancy. With bearing it in mind, our study has been focused on the comparative analysis of the LMP1 EBV polymorphism among the indigenous population of the Khabarovsk Territory (Nanai) and immigrants from the European part of the country to this region, which is not endemic for EBV-associated pathologies, but is located on the border with endemic EBV-associated form of nasopharyngeal carcinoma in southern provinces of China. The results obtained clearly showed sequences of LMP1 samples of the virus strains infecting Nanai and immigrants in the Khabarovsk Territory to be similar to LMP1 variants from different parts of the world previously described in the literature and have a number of unique mutation features.

About the authors

Ksenia V. Smirnova

N.N. Blokhin National Medical Cancer Research Center

Email: skv.lab@yandex.ru
MD, PhD, senior researcher of the Laboratory of Viral Carcinogenesis of the Research Institute of Carcinogenesis of the N.N. Blokhin National Medical Cancer Research Center, 24, Kashirskoe shosse, 115478 Moscow, Russia 24, Kashirskoe shosse, Moscow, 115478, Russia

S. V Diduk

N.N. Blokhin National Medical Cancer Research Center

науч. сотр. лаб. вирусного канцерогенеза НИИ канцерогенеза ФГБУ НМИЦ онкологии им. Н.Н. Блохина Минздрава России 24, Kashirskoe shosse, Moscow, 115478, Russia

V. E Gurtsevitch

N.N. Blokhin National Medical Cancer Research Center

вед. науч. сотр. лаб. вирусного канцерогенеза НИИ канцерогенеза ФГБУ НМИЦ онкологии им. Н.Н. Блохина Минздрава России. 24, Kashirskoe shosse, Moscow, 115478, Russia

References

  1. Young L.S., Rickinson A.B. Epstein-Barr virus: 40 years on. Nat. Rev. Cancer. 2004; 4(10): 757-68.
  2. Kaye K., Izumi K., Kieff E. Epstein-Barr virus latent membrane protein 1 is essential for B-lymphocyte growth transformation. Proc. Natl. Acad. Sci. USA. 1993; (90): 9150-4.
  3. Mainou B.A., Raab-Traub N. LMP1 strain variants: biological and molecular properties. J. Virol. 2006; (80): 6458-68.
  4. Fielding C.A., Sandvej K., Mehl A. et al. Epstein-Barr virus LMP-1 natural sequence variants differ in their potential to activate cellular signaling pathways. J. Virol. 2001; 75(19): 9129-41.
  5. Johnson R.J., Stack M., Hazlewood S.A. et al. The 30-base-pair deletion in chinese variants of the Epstein-Barr virus LMP1 gene is not the major effector of functional differences between variant LMP1 genes in human lymphocytes. J. Virol. 1998; 72(5): 4038-48.
  6. Blake S.M., Eliopoulos A.G., Dawson C.W., Young L.S. The transmembrane domains of the EBV-encoded latent membrane protein 1 (LMP1) variant Cao regulate enhanced signalling activity. Virology. 2001; (282): 278-87.
  7. Baichwal V.R., Sudgen B. Transformation of Balb3T3 by the BNLF-1 gene of Epstein-Barr virus. Oncogene. 1988; 2(5): 461-7.
  8. Knecht H., Bachmann E., Brousset P. et al. Deletions within the LMPl oncogene of Epstein-Barr virus are clustered in Hodgkin’s disease and identical to those observed in nasopharyngeal carcinoma. Blood. 1993; 82(10): 2937-42.
  9. Cheung S.-T., Lo K.-W., Leung S. et al. Prevalence of LMP1 deletion variant of Epstein-Barr virus in nasopharyngeal carcinoma and gastric tumors in Hong Kong. Int. J. Cancer. 1996; (66): 711-20.
  10. Dolcetti R., Zancai P., de Re V. et al. Epstein-Barr virus strains with latent membrane protein-1 deletions: prevalence in the Italian population and high association with human immunodeficiency virus-related Hodgkin’s disease. Blood. 1997; (89): 1723-31.
  11. Смирнова К.В., Дидук С.В., Гурцевич В.Э. Функциональный анализ вариантов латентного мембранного белка 1 (LMP1) вируса Эпштейна-Барр у больных лимфопролиферативными заболеваниями. Биомедицинская химия. 2011; 57(1): 114-26.
  12. Senuyuta N., Yakovleva L., Goncharova E. et al. Epstein-Barr virus latent membrane protein 1 (LMP-1) polymorphism in nasopharyngeal carcinoma and other oral cavity tumors in Russia. J. Medical Virology. 2014; 86(2): 290-300.
  13. Гончарова Е.В., Сенюта Н.Б., Смирнова К.В. и др. Вирус Эпштейна-Барр (ВЭБ) в России: инфицированность населения и генотипический анализ вариантов гена LMP1 у больных ВЭБ-ассоциированными патологиями и здоровых лиц. Вопросы вирусологии. 2015; 60(2): 11-7.
  14. Edwards R., Seillier Moisewitsch F., Raab-Traub N. Signature amino acids changes in latent membrane protein 1 distinguish Epstein-Barr virus strains. Virology. 1999; (261): 79-95.
  15. Mainou B.A., Raab-Traub N. LMP1 strain variants: biological and molecular properties. J. Virol. 2006; 80(13): 6458-68.
  16. Sandvej K., Gratama J.W., Munch M. et al. Sequence analysis of the Epstein-Barr virus (EBV) latent membrane protein-1 gene and promoter region: identification of 4 variants among wild-type EBV isolates. Blood. 1997; (90): 323-30.
  17. Walling D., Shebib N., Weaver S. et al. The molecular epidemiology and evolution of Epstein-Barr virus: sequence variation and genetic recombination in the latent membrane protein-1 gene. J. Infect. Dis. 1997; (179): 763-74.
  18. Sung N.S., Edwards R.H., Seillier-Moiseiwitsch F. et al. Epstein-Barr virus strain variation in nasopharyngeal carcinoma from the endemic and non-endemic regions of China. Int. J. Cancer. 1998; 76(2): 207-15.
  19. Burt R.D., Vaughan T.L., Mcknight B. et al. Associations between human leukocyte antigen type and nasopharyngeal carcinoma in Caucasians in the United States. Cancer Epidemiol. Biomarkers Prev. 1996; 5(11): 879-87.
  20. Hildesheim A., Apple R.J., Chen C.J. et al. Association of HLA class I and II alleles and extended haplotypes with nasopharyngeal carcinoma in Taiwan. J. Natl. Cancer Inst. 2002; 94(23): 1780-9.
  21. Ward M.H., Pan W.H., Cheng Y.J. et al. Dietary exposure to nitrite and nitrosamines and risk of nasopharyngeal carcinoma in Taiwan. Int. J. Cancer. 2000; 86(5): 603-9.
  22. Vaughan T.L., Stewart P.A., Teschke K. et al. Occupational exposure to formaldehyde and wood dust and nasopharyngeal carcinoma. Occup. Environ. Med. 2000; 57(6): 376-84.
  23. Давыдов М.И., Аксель Е.М. Статистика злокачественных новообразований в России и странах СНГ в 2012 году. М.; Издательская группа РОНЦ; 2014.
  24. Miller W.E., Edwards R.H., Walling D.M., Raab-Traub N. Sequence variation in the Epstein-Barr virus latent membrane protein. J. Gen. Virol. 1994; (75): 2729-40.
  25. Li S.N., Chang Y.S., Liu S.T. Effect of a 10 Aamino acid deletion on the oncogenic activity of latent membrane protein 1 of Epstein-Barr virus. Oncogene. 1996; (12): 2129-35.
  26. Nagamine M., Takahara M., Kishibe K. et al. Sequence variations of Epstein-Barr virus LMP1 gene in nasal NK/T cell lymphoma. Virus Genes. 2007; (34): 47-54.
  27. Walling D.M., Raab-Traub N. Epstein-Barr virus intrastrain recombination in oral hairy leukoplakia. J. Virol. 1994; 68(12): 7909-17.
  28. Burrows J.M., Burrows S.R., Poulsen L.M. et al. Unusually high frequency of Epstein-Barr virus genetic variants in Papua New Guinea that can escape cytotoxic T-cell recognition: implications for virus evolution. J. Virol. 1996; 70(4): 2490-6.

Copyright (c) 2017 Eco-vector


 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies