The use of human placenta for purification of normal and immune animal sera


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Abstract

There was described and efficient and economical approach for the removal of toxic substances from normal and immune sera from various species of animals with the use of human placenta tissue. Purification brings about to perceptible losses of neither serum-specific activity nor the original volume. Being simple the method does not require any special equipment and can be used in conditions of low-volume or in laboratory production of serum preparations. There are considered as well possible origins of serum toxicity as mechanism of antitoxic activity of placenta.

About the authors

V. G Kozlov

ФГУП «Предприятие по производству бактерийных и вирусных препаратов Института полиомиелита и вирусных энцефалитов им. М.П. Чумакова»

Email: vgkozlov@mail.ru
канд. мед. наук, зав отд. диагностических препаратов 142782 РФ, г. Москва, поселение Московский, поселок Института полиомиелита

Yu. Yu Ivin

Chumakov Institute of Poliomyelitis and Viral Encephalitides

мл. науч. сотр. 27 km of Kievskoye shosse, village of the Institute of Poliomyelitis, settlement Moskovsky, Moscow, Russian Federation, 142782

V. P Grachev

Chumakov Institute of Poliomyelitis and Viral Encephalitides

доктор биол. наук, проф., гл. науч. сотр. 27 km of Kievskoye shosse, village of the Institute of Poliomyelitis, settlement Moskovsky, Moscow, Russian Federation, 142782

References

  1. Козлов В.Г., Викторова Е.Г., Набатников П.А.. Цитотоксические свойства кроличьих энтеровирусных диагностических сывороток. Особенности и локализация. Вопросы вирусологии. 2009, 1: 22-7.
  2. Рекомендации по эпидемиологическому надзору за энтеровирусами для поддержки программы ликвидации полиомиелита. Женева. ВОЗ. 2005.
  3. Вolande R.P., Arnold D.C. Natural cytotoxicity of human serum. A natural IgM antibody sensitizes transformed murine cells to lytic action of complement. Pathol. Immunopathol. Res. 1989, 8: 46-60.
  4. Colley D.G., Waksman B.H.. Cytotoxic effect of normal rabbit serum on rat iymphoid cеlls. Trasplantation. 1970; 96: 395-401.
  5. Fedoroff S., Cook B. Interaction between cells and cytotoxic sera in cell cultures. Pathol. Biol. 1961, 9: 584-90.
  6. Козлов В.Г. Поликлональные энтеровирусные диагностические сыворотки нового поколения. Эпидемиология и инфекционные болезни. 2014; 3: 51-6.
  7. Козлов В.Г., Викторова Е.Г. Оценка эффективности и реактогенности эмульсионных адъювантных систем при изготовлении поликлональных энтеровирусных диагностических сывороток. Вопросы вирусологии. 2011; 2: 41-6.
  8. Doherty P.C. Cell-mediated cytotoxity. Cell. 1993, 75: 607-12.
  9. Карпов С.П., Прегер С.М., Синельников Г.Е., Федоров Ю.В. Гипериммунные сыворотки. Томск; 1976: 82-90.
  10. Buhimschi I.A., Jabr M., Buhimschi C.S. et al. The novel antimicrobial peptide beta3-defensin is produced by the amnion: a possible role of the fetal membranes in innate immunity of the amniotic cavity. Am. J. Obstet. Gynecol. 2004; 191: 1678-87.
  11. King A.E., Paltoo A., Kelly R.W. et al. Expression of natural antimicrobials by human placenta and fetal membranes. Placenta. 2007; 28: 161-9.
  12. Bolande R.P., Mayer D. The cytolisis of human neuroblastoma cell by natural IgM antibody-complement system in pregnancy serum. Cancer Invest. 1990, 8: 603-11.
  13. Ollert M.W., David K., Schmitt C. et al. Normal human serum contains a natural IgM antibody cytotoxic for human neuroblastoma cells. Proc. Natl Acad. Sci. USA. 1996; 93: 4498-503.
  14. McNabb T., Koh T.Y., Dorrington K.J., Painter R.H.. Structure and function of immunoglobulin domains. V. Binding of immunoglobulin G on fragments to placental membrane preparations. J. Immunol. 1976, 117: 882-8.

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