APPLICATION OF ELISA-IGM (MAC-ELISA) FOR DETERMINING ETIOLOGICAL LINK VIRUS TYPES IN CONCRET CASES OF THE DISEASE


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Abstract

105 sera from 101 patients with a confirmed diagnosis of dengue fever (LD) were examined using monospecific ELISA-IgM test. Monospecific results were observed in 39 samples (37.1%). 27 sample of them were taken during the first 7 days of the disease. In those systems were examined 23 serum samples of 23 patients, in which etiological role of one of the four dengue viruses was been established by the RT-PCR method. In this case, the coincidence-IgM ELISA results and the RT-PCR took place in 14 sera (60.9%).

About the authors

Yuliya A. Akinshina

ZAO «ECOlab»

Email: akinshina.opr@mail.ru
microbiologist of development department of ZAO «EСOlab» 142530, Electrogorsk, Russion Federation

V. F Larichev

The N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, The D.I.Ivanovsky Institute of Virology

Email: arboelisa@mail.ru
доктор мед. наук, вед. науч. сотр. ФГБУ «Федеральный научно-исследовательский центр эпидемиологии и микробиологии имени почетного академика Н.Ф. Гамалеи», «НИИ вирусологии им. Д.И. Ивановского» Минздрава России 12309818, Moscow, Russion Federation

M. A Sayfullin

Сlinical Infectious Diseases Hospital № 1 of the Department of Health care of Moskow

Email: dr_saifullin@mail.ru
врач-инфекционист, зав. отделением ГБУЗ «Инфекционная клиническая больница № 1» ДЗМ 123367, Moscow, Russion Federation

S. G Mardanly

ZAO «ECOlab»

Email: ekolab-president@mail.ru
доктор мед. наук, проф. каф. фармакологии и фармацевтических дисциплин ГОУ ВО МО «Государственный гуманитарно-технологический университет», президент и директор по науке ЗАО «ЭКОлаб» 142530, Electrogorsk, Russion Federation

A. M Butenko

The N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, The D.I.Ivanovsky Institute of Virology

12309818, Moscow, Russion Federation

References

  1. ВОЗ. Денге и тяжелая денге. Информационный бюллетень. 2015; (117).
  2. Weiskopf D., Angelo M.A., Sidney J., Peters B., Shresta S., Sette A. Immunodominance changes as a function of the infecting dengue virus serotype and primary versus secondary infection. J. Virol. 2014; 88 (19): 11 383-94.
  3. Ларичев В.Ф., Сайфуллин М.А., Акиншина Ю.А., Хуторецкая Н.В., Бутенко А.М. Завозные случаи арбовирусных инфекций в Российской Федерации. Эпидемиол. и инфекц. бол. 2012; (1): 35-9.
  4. Akehurst C. Dengue virus genotypes and haemorrhagic fever. Eurosurveillance. 1999; 3 (44): pii=1309.
  5. Kochel T.J., Watts D.M., Gozalo A.S., Ewing D.F., Porter K.R., Russell K.L. Cross-serotype neutralization of dengue virus in aotus nancymae monkeys. J. Infect. Dis. 2005; 191 (6): 1000-4.
  6. Vorndam V., Kuno G., Gubler D.J., Kuno G. Dengue and Dengue Hemorrhagic Fever. New York: CAB International. Laboratory Diagnosis of Dengue Virus Infections; 1997: 313-33.
  7. Najioullah F., Viron F., Césaire R. Evaluation of four commercial real-time RT-PCR kits for the detection of dengue viruses in clinical samples. Virol. J. 2014; 11 (1): 164.
  8. WHO. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control. New Edition. 2009. ISBN 978-9-241547-87-1

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