COMPARISON OF THE APPLICATION OF DOMESTIC «ELISA-IGM-DENGUE» KIT, DELIVERED IN THE D.I. IVANOVSKY INSTITUTE OF VIROLOGY (MOSCOW, RUSSIAN FEDERATION) AND «ANTI-DENGUE VIRUS ELISA IGM» KIT (EUROIMMUN, GERMANY) FOR THE SERODIAGNOSIS OF DENGUE FEVER


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Abstract

88 sera from patients with dengue fever were examined with the use of two test systems: Pilot Production kit «ELISA- IgM-dengue», delivered in the D.I. Ivanovsky Institute of Virology (Russian Federation) and. All 88 samples were positive for specific IgM in «ELISA-IgM-dengue», but 8 cases examined with the test kit «Anti-Dengue virus ELISA (IgM)» (Euroimmun, Germany) appeared to be negative. The ratio between the titers of anti-dengue IgM determined in patients’ blood samples by "ELISA-IgM-dengue" and «Ratio» (index, recommended by the company «Euroimmun» for differentiation of positive, equivocal or negative results, was evaluated. 53 blood sample containing M antibodies to cytomegalovirus (n = 43), Epstein-Barr virus (n = 6), herpes simplex virus (n = 2) and rubella virus (n = 2) were tested by both ELISA kits to compare their specificity. When «ELISA- IgM-dengue» kits were applied all observed samples were negative, but under the application of the «Anti-Dengue virus ELISA (IgM)» kit (Euroimmun, Germany) 17 samples (32.1%) were false positive or equivocal.

About the authors

Yu. A Akinshina

ZAO «ECOlab»

Email: akinshina.opr@mail.ru
1, Budennogo str., Electrogorsk, 142530, Russian Federation

V. F Larichev

N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, The D.I. Ivanovsky Institute of Virology

Email: arboelisa@mail.ru
18, Gamalei str., Moscow, 123098, Russian Federation

M. A Saifullin

Сlinical Infectious Diseases Hospital No 1

Email: dr_saifullin@mail.ru
63, Volokolamskoye Sh., Moscow, 123367, Russian Federation

S. G Mardanly

ZAO «ECOlab»

Email: ekolab-president@mail.ru
1, Budennogo str., Electrogorsk, 142530, Russian Federation

A. M Butenko

N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, The D.I. Ivanovsky Institute of Virology

18, Gamalei str., Moscow, 123098, Russian Federation

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