Molecular genetic characterization of Vibrio cholerae non-01/non-0139 strains isolated from ship ballast and port surface water in rostov region


Cite item

Full Text

Abstract

In the given paper there are presented the results of analysis for presence in genomes of Vibrio cholerae non-O1/non-O139 strains isolated from ships' ballast water and the coastal zone of Taganrog Gulf class 1 integrons (In1) and SXT/R391 integrative conjugative elements. The intact sequence of In13'-conservative segment (qacEdelta1-sul) was detected in 3 strains (no. 33, 59, 182), whereas in strains V. cholerae no. 28 and 52 there were revealed the probable changes of target sequence (duplication and deletion, respectively). The fragment of SXT/R391 integrase IntSXT gene was detected in 7 of 10 strains tested. Group of strains no. 27, 36, 38, 117 and 59, 182 belong to 2 most common identified genotypes, while the rest isolates are characterized by unique genotypes that indicates to different source of their origins.

About the authors

I. B Zakharova

Volgograd Anti-Plague Research Institute of Federal Service on Customers' Rights Protection and Human Well-being Surveillance

7, Golubinskaya Str., Volgograd, Russian Federation, 400131

S. Yu Vodyanitskaya

Rostov-on-Don Institute for Plague Control of Federal Agency for Consumer Rights Protection and Human Welfare Supervision

117/40, M. Gorkogo Str., Rostov-on-Don, Russian Federation, 344002

M. V Podshivalova

Volgograd Anti-Plague Research Institute of Federal Service on Customers' Rights Protection and Human Well-being Surveillance

7, Golubinskaya Str., Volgograd, Russian Federation, 400131

V. D Kruglikov

Rostov-on-Don Institute for Plague Control of Federal Agency for Consumer Rights Protection and Human Welfare Supervision

117/40, M. Gorkogo Str., Rostov-on-Don, Russian Federation, 344002

I. V Arkhangelskaya

Rostov-on-Don Institute for Plague Control of Federal Agency for Consumer Rights Protection and Human Welfare Supervision

117/40, M. Gorkogo Str., Rostov-on-Don, Russian Federation, 344002

D. V Viktorov

Volgograd Anti-Plague Research Institute of Federal Service on Customers' Rights Protection and Human Well-being Surveillance

7, Golubinskaya Str., Volgograd, Russian Federation, 400131

References

  1. Rodríguez-Blanco A., Lemos M., Osorio C. Integrating conjugative elements as vectors of antibiotic, mercury, and quaternary ammonium compound resistance in marine aquaculture environments. Antimicrob. Agents Chemother. 2012; 56(5): 2619-26.
  2. Водяницкая С.Ю., Лях О.В. Разработка способов отбора балластной воды на судах смешанного «река-море» плавания для исследования на холеру. Здоровье населения и среда обитания. 2014; 1: 37-40.
  3. Методические указания «Лабораторная диагностика холеры (МУК 4.2.2218-07)». М.; 2007.
  4. Mazel D. Integrons: agents of bacterial evolution. Nature Rev. Microbiol. 2006; 4: 608-20.
  5. Wozniak R., Fouts D., Spagnoletti M., et al. Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs. PLoS Genet. 2009; 5 (12): e1000786. doi: 10.1371/journal.pgen.1000786.
  6. Подшивалова М.В., Кузютина Ю.А., Захарова И.Б., Лопастейская Я.А., Викторов Д.В. Характеристика антибиотикорезистентных штаммов Vibrio cholerae, несущих интегративные конъюгативные элементы SXT типа. Эпидемиология и инфекционные болезни. 2014; 18 (3): 34-9.
  7. Сустретова Н.В. Обеспечение экологической безопасности балластных вод на судах смешанного плавания «река-море»: Дисс. ... канд. техн. наук. Н. Новгород; 2011.
  8. Водяницкая С.Ю., Лях О.В., Ломов Ю.М., Рыжков Ю.В., Иванова Н.Г., Иванова А.И. и др. Противоэпидемические (профилактические) мероприятия при выделении возбудителей холеры из судовых балластных вод. Эпидемиология и вакцинопрофилактика. 2014; 2 (75): 40-4.

Copyright (c) 2015 Eco-vector


 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies