The dynamics of HCV DNA and HBsAg levels in acute hepatitis B patients in monoinfection and coinfection of other hepatitis viruses


Cite item

Full Text

Abstract

Introduction Changes in the levels of DNA of hepatitis B virus (HBV) infection and the quantitative content of the surface antigen (HBsAg) in acute hepatitis B, as well as the concomitant co-injection of other hepatitis viruses have been insufficiently studied so far. Materials and Methods In 21 patients with acute HBV monoinfection and 27 patients with co-infection of HBV + HCV and/ or HDV three to four serum samples were withdrawn with interval of 6-10 days and the dynamics of HBV viral load and concentration of HBsAg was examined. Results Logarithm of the concentration of DNA HBV was established to decrease from 4.0 ± 0.65 to 3.0 ± 0.59, to 2.5 ± 0.47, to 1.9 ± 0.65 (IU /ml, M ± s), the half-life of DNA HBV was increased from 1.6 days at baseline to 4 days to the end of the study. The decline of the HBsA concentration proceeded much slower: from 38 to 23, 12, 3.8 pg/ml. Half-life period of HBsAg accounted of 8 days at the beginning and 5.7 days at the end of the study, however, in 11 patients there was observed rapid elimination of HBsAg and disappearance of HBV DNA. Conclusion The fact of co-infection with hepatitis C virus (HCV) had no significant effect on the dynamics of HBV DNA and HBsAg levels. In patients infected with Hepatitis D virus (HDV) HBV viral load was significantly lower, and the concentration of HBsAg, by contrast, is significantly higher than in acute monoinfection with HBV. Thus, in acute hepatitis B DNA elimination occurs much faster than the elimination of HBsAg. In addition, in co-infection HDV inhibits replication of HBV, but at the same time stimulates the expression of HBsAg. The obtained data should be considered in the interpretation of laboratory tests in patients with acute hepatitis B, both in the earlier period, and at follow-up.

About the authors

Vladimir P. Chulanov

I.M. Sechenov First Moscow State Medical University; Central Research Institute of Epidemiology of Federal Service on Customers’ Rights Protection and Human Well-Being Surveillance

Email: vladimir.chulanov@pcr.ru
MD., PhD., DSci., head of the Center for Clinical Diagnostics and Research, head of the Reference Centre for Monitoring of Viral Hepatitis, Central Research Institute of Epidemiology of Clinical Diagnostics and Research 8/2, Trubetskaya str., Moscow, 119992, Russian Federation

E. Yu Kostygova

I.M. Sechenov First Moscow State Medical University

8/2, Trubetskaya str., Moscow, 119992, Russian Federation

D. S Kostyushev

Central Research Institute of Epidemiology of Federal Service on Customers’ Rights Protection and Human Well-Being Surveillance

3а, Novogireevskaya, Moscow, 111123, Russian Federation

D. Glebe

University of Giessen

Institute of Medical Virology 23, Ludwigstrafie, Giessen, 35392, Germany

G. A Shipulin

Central Research Institute of Epidemiology of Federal Service on Customers’ Rights Protection and Human Well-Being Surveillance

3а, Novogireevskaya, Moscow, 111123, Russian Federation

V. Gerlikh

University of Giessen

Institute of Medical Virology 23, Ludwigstrafie, Giessen, 35392, Germany

E. V Volchkova

I.M. Sechenov First Moscow State Medical University

8/2, Trubetskaya str., Moscow, 119992, Russian Federation

References

  1. Симонова И.А., Волчкова Е.В., Чуланов В.П. и др. Алгоритм специфической лабораторной диагностики вирусных гепатитов. Клин. лаб. диагн. 2000; 8: 21-33.
  2. Erhardt A., Reineke U., Blondin D. et al. Mutations of the core promoter and response to interferon treatment in chronic replicative hepatitis B. Hepatology. 2000; 31: 716-25.
  3. Schaefer S., Glebe D., Wend U.C. et al. Universal primers for real-time amplification of DNA from all known Orthohepadnavirus species. J. Clin. Virol. 2003; 27: 30-7.
  4. Saldanha J., Gerlich W., Lelie N. et al. An international collaborative study to establish a World Health Organization international standard for hepatitis B virus DNA nucleic acid amplification techniques. WHO Collaborative Study Group. Vox Sang. 2001; 80 (1): 63-71.
  5. Williams V., Brichler S., Radjef N. et al. Hepatitis delta virus proteins repress hepatitis B virus enhancers and activate the alpha/beta interferon-inducible MxA gene. J. Gen. Virol. 2009; 90 (11): 2759-67. doi: 10.1099/vir.0.011239-0. Epub 2009 Jul 22.
  6. Yu G., Chi X., Wu R. et al. Replication inhibition of hepatitis B virus and hepatitis C virus in co-infected patients in Сhinese population. PLoS One. 2015; 10(9):e0139015. doi: 10.1371/journal.pone.0139015. eCollection 2015.
  7. Шкурко Т.В., Чешик С.Г. Гепатит В у анти-НСV-позитивных пациентов. Вопр. вирусол. 2000; 45 (3): 32-5.
  8. Fong T.L., Di Bisceglie A.M., Biswas R. et al. High levels of viral replication during acute hepatitis B infection predict progression to chronicity. J. Med. Virol. 1994; 43: 155-8.
  9. Whalley S.A., Murray J.M., Brown D. et al. Kinetics of acute hepatitis B virus infection in humans. J. Exp. Med. 2001; 193: 847-54.
  10. Nowak M.A., Bonhoeffer S., Hill A.M. et al. Viral dynamics in hepatitis B virus infection. Proc. Natl. Acad. Sci. USA. 1996; 93 (9): 4398-402.
  11. Tsiang M., Rooney J.F., Toole J.J., Gibbs C.S. Biphasic clearance kinetics of hepatitis B virus from patients during adefovir dipivoxil therapy. Hepatology. 1999; 29 (6): 1863-9.
  12. Günal Ö., Barut Ş., Etikan İ. et al. Relation between serum quantitative HBsAg, ALT and HBV DNA levels in HBeAg negative chronic HBV infection. Turk. J. Gastroenterol. 2014; 25 (Suppl. 1): 142-6. doi: 10.5152/tjg.2014.5711.
  13. Böcher W.O., Herzog-Hauff S., Schlaak J. et al. Kinetics of hepatitis B surface antigen-specific immune responses in acute and chronic hepatitis B or after HBs vaccination: stimulation of the in vitro antibody response by interferon gamma. Hepatology. 1999; 29 (1): 238-44.
  14. Dunn M. et al. The disappearance rate of glutamic oxaloacetic transaminase from the circulation and its distribution in the body’s fluid compartments and secretions. J. Lab. Clin. Med. 1958; 51: 259.

Copyright (c) 2016 Eco-vector


 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies