Assessment of a new "DS-EIA-HIV-AB-TERM" assay to determine the probable time of infection with human immunodeficiency virus-1 (HIV-1)


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Abstract

The epidemiological situation for HIV infection in Russia remains to be extremely stringent, which requires a search for new, more efficient and cost-effective solutions aimed at countering the epidemic. The inclusion of laboratory studies with the use of a test system for the determination of the remoteness of HIV infection in routine surveillance practices will permit to determine the frequency of occurrence of early cases of infections among newly diagnosed HIV-positive persons. The analysis of this criterion at the population level will help to objectively evaluate the effectiveness of prevention, control activities carried out in a particular area. The increase of objectivity in the determination of the time elapsed from the moment of infection of HIV-1 with the use of the test system DSEIA-HIV-Ab-TERM provides the opportunity to use the obtained results in the epidemiological investigation for the purpose of more accurate and complete identification of contact persons. The determination of the probable period of infection may also be required in the solving the question on the prescription of antiretroviral therapy as a complementary study in the monitoring of HIV drug resistance. The aim of this work was to determine the effectiveness of the new test system DS-EIAHIV-Ab-TERM for the determination of the likely timing of HIV-1 infection. There were studied serum samples (plasma) of blood of HIV-infected persons with epidemiologically established time of infection (n = 281) and samples of commercial seroconversion panels. Results of the performed study showed that the probability of a correct detection of the remoteness of HIV-1 infection for samples from individuals with the most probable time of the established fact of infection was 95%, for samples of commercial seroconversion panels - 100%. The data demonstrated the high efficacy of the test system DS-“EIAHIV-Ab-TERM” in the determination of the most probable timing of infection with human immunodeficiency virus type 1, that in combination with the speed and simplicity of the procedure allows to recommend it for use in the laboratory practice.

About the authors

Yu. E Zagryadskaya

NPO “Diagnostic Systems"

Email: ZagryadskayaUE@npods.ru
22, Yablonevaya Str. , Nizhniy Novgorod, Russian Federation, 603093

D. A Neshumaev

Krasnoyarsk Regional Centre of AIDS Prevention

канд. мед. наук, врач клинической лабораторной диагностики лаборатории бактериологических и молекулярно-генетических исследований 45, Karla Marksa Str., Krasnoyarsk, Russian Federation, 660049

Yu. A Kokotyukha

Krasnoyarsk Regional Centre of AIDS Prevention

врач клинической лабораторной диагностики лаборатории иммуноферментных исследований 45, Karla Marksa Str., Krasnoyarsk, Russian Federation, 660049

E. M Meyrmanova

Krasnoyarsk Regional Centre of AIDS Prevention

врач клинической лабораторной диагностики лаборатории иммуноферментных исследований 45, Karla Marksa Str., Krasnoyarsk, Russian Federation, 660049

I. A Olkhovskiy

Hematological Scientific Centre

Krasnoyarsk branch 50, Akademgorodok, Krasnoyarsk, Russian Federation, 660036

V. F Puzyrev

NPO “Diagnostic Systems"

канд. биол. наук, нач. отд. производства рекомбинантных антигенов и конъюгатов 22, Yablonevaya Str. , Nizhniy Novgorod, Russian Federation, 603093

A. N Burkov

NPO “Diagnostic Systems"

доктор мед. наук, проф., генеральный директор 22, Yablonevaya Str. , Nizhniy Novgorod, Russian Federation, 603093

T. I Ulanova

NPO “Diagnostic Systems"

доктор биол. наук, директор по научной работе 22, Yablonevaya Str. , Nizhniy Novgorod, Russian Federation, 603093

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