Features of the replication of hantaviruses - pathogens of hemorrhagic fever with renal syndrome in cell cultures of different origin


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Abstract

There are presented results of the adaptation ofpathogens of hemorrhagic fever with renal syndrome (HFRS) virus Puumala and Dobrava strains, to the reproduction in cell cultures of the simian origin - Green monkey kidney (Vero, 4647), Green monkey spleen (455), calf kidney (PT-1) and sheep kidney (4184). Hantaviruses were shown to be able to adapt to the replication in the new cellular system differing in species origin from the culture of Vero E6 origin in which the strains were isolated. This fact demonstrates the heterogeneity of hantavirus population, with the existence of «quasispecies» which can recognize and bind to ligands (b3 integrins) of the other species specificity. The highest yield of the virus, which is comparable with the culture of Vero E6, culture Vero provides. In Vero cells after 3-4 passages Puumala and Dobrava-Belgrade viruses replicate also actively as in a permissive culture Vero E6, that proposes the use of Vero culture cell allowed for the production of immunobiological drugs administered to people as a substrate in the delivery of vaccines against HFRS.

About the authors

G. A Malkin

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides

Email: centrglps@rambler.ru
мл. науч. сотр. лаб. геморрагических лихорадок 27@th km. of Kiev Highway, Leninsky District, Moscow Region, Russian Federation, 142782

T. K Dzagurova

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides

Email: centrglps@rambler.ru
канд. мед. наук, зав. лаб. геморрагических лихорадок 27@th km. of Kiev Highway, Leninsky District, Moscow Region, Russian Federation, 142782

N. A Korotina

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides

Email: centrglps@rambler.ru
науч. сотр. лаб. геморрагических лихорадок 27@th km. of Kiev Highway, Leninsky District, Moscow Region, Russian Federation, 142782

M. V Balovneva

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides

Email: centrglps@rambler.ru
канд. мед. наук., ст. науч. сотр. лаб. геморрагических лихорадок 27@th km. of Kiev Highway, Leninsky District, Moscow Region, Russian Federation, 142782

O. I Konyushko

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides

Email: polcells@yandex.ru
канд. биол. наук., ст. науч. сотр. лаб. иммунологии и культур тканей 27@th km. of Kiev Highway, Leninsky District, Moscow Region, Russian Federation, 142782

S. E Sotskova

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides

Email: se-sotskova@mail.ru
руководитель отдела биологической безопасности 27@th km. of Kiev Highway, Leninsky District, Moscow Region, Russian Federation, 142782

E. A Tkachenko

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides

Email: evgeniytkach@mtu-net.ru
доктор мед. наук, проф., зам. директора по науке 27@th km. of Kiev Highway, Leninsky District, Moscow Region, Russian Federation, 142782

References

  1. Ткаченко Е.А., Дзагурова Т.К., Ткаченко П.Е. Хантавирусы: экология, молекулярная биология, морфология, патогенез и диагностика хантавирусных инфекций. Молекулярная медицина. 2009; 5: 36-41.
  2. Plyusnin A., Morzunov S.P. Virus evolution and genetic diversity of hantaviruses and their rodent hosts. Curr. Top. Microbiol. Immunol. 2001; 256: 47-75.
  3. Смородинцев А.А., Альтшуллер И.С., Дунаевский М.И. и др. Этиология и клиника геморрагического нефрозо-нефрита. М.: ВИЭМ, 1944.
  4. Hooper J., Larsen T., Custer D. et al. A lethal disease model for hantavirus pulmonary syndrome. Virology. 2001; 289: 6-14.
  5. French G.R., Foulke R.S., Brand О.А., et al. Korean hemorrhagic fever: propagation of the etiologic agent in a cell line of human origin. Science. 1981; 211: 1046-8.
  6. Pensiero M.N., Sharefkin J.B., Dieffenbach C.W., et al. Hantaan virus infection of human endothelial cells. J. Virol. 1992; 66: 5929-36.
  7. Yanagihara R., Silverman D.J. Fxperimental infection of human vascular endothelial cells by pathogenic and nonpathogenic hantaviruses. Arch. Virol. 1990; 111: 281-6.
  8. Nagai T., Tanishita О., Takahashi Y. et al. Isolation of haemorrhagic fever with renal syndrome virus from leukocytes of rats and virus replication in cultures of rat and human macrophages. J. Gen. Virol. 1985; 66: 1271-8.
  9. Temonen M., Vapalahti О., Holthofer H. et al. Susceptibility of human cells to Puumala virus infection. J. Gen. Virol. 1993; 74: 515-8.
  10. Song G., Huang Y.C., Hang C.S. Preliminary human trial of inactivated golden hamster kidney cell vaccine against HFRS. Vaccine. 1992; 10: 214-6.
  11. Ogino M., Yoshimatsu K., Fbihara H. et al. Cell fusion activities of hantaan virus envelope glycoproteins. J. Virology. 2004; 78 (19): 10776-82.
  12. Миронова Л.Л., Попова В.Д., Конюшко О.И. и др. Опыт создания банка авторских линий перевиваемых клеток и их применение в вирусологической практике. Биотехнология. 2000; 6: 41-7.
  13. Дзагурова Т.К., Ткаченко Е.А., Башкирцев В.Н. и др. Выделение и идентификация штаммов хантавирусов-возбудителей ГЛПС в европейской части России. В кн.: Медицинская вирусология. М.; 2008; 26: 142-50.
  14. Дзагурова Т.К., Солопова О.Н., Свешников П.Г. и др. Разработка иммуноферментной тест-системы на основе моноклональных антител для определения специфической активности вакцины против геморрагической лихорадки с почечным синдромом. Вопросы вирусологии. 2013; 58 (1): 40-4.
  15. Kruger D., Klempa B. Molecular detection of human viral pathogens. CRC Press; 2012: 631-38.
  16. Gavrilovskaya I.N., Brown E.J., Ginsberg M.H. et al. Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by b3 integrins. J. Virol. 1999; 73 (5): 3951-9.
  17. Muranyi W., Kehm R., Bahr U. et al. Bovine aortic endothelial cells are susceptible to hantavirus infection; a new aspect in hantavirus ecology. Virology. 2004; 318: 112-22.
  18. Иванов Л.И., Здановская Н.И., Дзагурова Т.К. и др. Адаптационная способность хантавирусов при изоляции на культуре клеток VERO-E6 в зависимости от вида экологического хозяина. Медицинская паразитология и паразитарные болезни. 2000; 4: 22-5.
  19. Kraus А.А., Raftery M.J., Giese T. et al. Differential antiviral response of endothelial cells after infection with pathogenic and non-pathogenic hantaviruses. J. Virology. 2004; 78 (12): 6143-50.
  20. Desmyter J., Melnick J.L., Rawls W.E. Defectiveness of interferon production and of rubella virus interference in a line of african green monkey kidney cells (Vero). J. Virology. 1968; 2 (10): 955-61.

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