Email this article Distribution of the causative agent of relapsing tick-borne fever Borrelia miyamotoi in natural focus in the Tomsk region
- Authors: Voronkova O.V.1, Lukashova L.V.1, Karpova M.R.1, Ilyinskikh E.N.1, Semenov A.G.1, Esimova I.E.1, Motlokhova E.A.1, Chernyshov N.A.1, Ilyanova I.N.1
-
Affiliations:
- Siberian State Medical University
- Issue: Vol 27, No 1 (2022)
- Pages: 15-22
- Section: ORIGINAL STUDIES
- URL: https://journals.rcsi.science/1560-9529/article/view/109070
- DOI: https://doi.org/10.17816/EID109070
- ID: 109070
Cite item
Open Access
Access granted
Subscription Access
Abstract
BACKGROUND: Knowledge about the infectious potential of the causative agent of recurrent tick-borne fever Borrelia miyamotoi and the role of this pathogen in the etiological structure formation of transmissible natural focal infections in a number of regions remains incomplete. Among them is the Tomsk region where in 2021 the incidence of ixodic tick-borne borreliosis was 15.7 per 100,000 population, which is 3.6 times higher than the all-Russian indicator.
AIMS: This study aimed to summarize and analyze epidemiological data, as well as the results of our own clinical observations and laboratory studies conducted in 2015–2021 confirming the distribution of the causative agent of relapsing tick-borne fever B. miyamotoi in the Tomsk region.
MATERIALS AND METHODS: Epidemiological data on the manifestation of the natural foci of tick-borne infections were analyzed in the Tomsk region for the period 2015–2021. A clinical case of febrile infection caused by B. miyamotoi infection is described as an example of underdiagnosis of relapsing tick-borne fever. Verification of the etiological variant of borreliosis was carried out using real-time PCR. DNA extraction and detection of B. miyamotoi genetic markers were performed using RealBest series kits (Vector-Best, Novosibirsk), followed by nucleic acid sequencing of B. miyamotoi 23SrRNA, glpQ, and recA gene sequences. Ixodid ticks (Ixodes persulcatus, Ixodes pavlovskyi, Dermacentor reticulatus) collected from the Tomsk region were studied for infestation with B. miyamotoi. The detection of genetic markers of pathogenic Borrelia was carried out in nucleic acid samples isolated from tick homogenates using kits of the RealBest series (Vector-Best, Novosibirsk).
RESULTS: In 2016, the first clinical case of borreliosis caused by B. miyamotoi was confirmed by molecular genetic methods in the Tomsk region. During the period 2015–2021, the infection rate of B. miyamotoi ticks of the genus Ixodes in the Tomsk region was up to 7%, ticks of the genus Dermacentor ― up to 3%.
CONCLUSION: The results of this study demonstrated the distribution of the causative agent of relapsing tick-borne fever B. miyamotoi in the natural foci of the Tomsk region, which requires further monitoring of the epizootic situation and study of the role of this pathogen in the formation of the etiological structure of transmission natural focal infections.
Full Text
##article.viewOnOriginalSite##About the authors
Olga V. Voronkova
Siberian State Medical University
Email: Voronkova-ov@yandex.ru
ORCID iD: 0000-0001-9478-3429
SPIN-code: 8005-8110
MD, Dr. Sci. (Med.), Associate Professor
Russian Federation, 2, Moscowski trakt, Tomsk, 634050Larisa V. Lukashova
Siberian State Medical University
Email: luni-april@yandex.ru
ORCID iD: 0000-0001-7119-391X
SPIN-code: 8196-9335
MD, Dr. Sci. (Med.), Associate Professor
Russian Federation, 2, Moscowski trakt, Tomsk, 634050Maria R. Karpova
Siberian State Medical University
Email: mrkarpova@mail.ru
ORCID iD: 0000-0002-7109-9955
SPIN-code: 6935-7447
MD, Dr. Sci. (Med.), Professor
Russian Federation, 2, Moscowski trakt, Tomsk, 634050Ekaterina N. Ilyinskikh
Siberian State Medical University
Email: infconf2009@mail.ru
ORCID iD: 0000-0001-7646-6905
SPIN-code: 5245-5958
MD, Dr. Sci. (Med.), Associate Professor
Russian Federation, 2, Moscowski trakt, Tomsk, 634050Albert G. Semenov
Siberian State Medical University
Email: albertgs@yandex.ru
ORCID iD: 0000-0002-6382-8548
SPIN-code: 2003-5281
Russian Federation, 2, Moscowski trakt, Tomsk, 634050
Irina E. Esimova
Siberian State Medical University
Email: orevi@mail.ru
ORCID iD: 0000-0002-7508-2878
SPIN-code: 2245-6398
MD, Dr. Sci. (Med.)
Russian Federation, 2, Moscowski trakt, Tomsk, 634050Elizaveta A. Motlokhova
Siberian State Medical University
Email: emotlohova@gmail.com
ORCID iD: 0000-0001-7409-3770
SPIN-code: 1166-6131
Russian Federation, 2, Moscowski trakt, Tomsk, 634050
Nikita A. Chernyshov
Siberian State Medical University
Email: niki-rembo@mail.ru
ORCID iD: 0000-0002-4008-5606
SPIN-code: 7863-9900
Russian Federation, 2, Moscowski trakt, Tomsk, 634050
Irina N. Ilyanova
Siberian State Medical University
Author for correspondence.
Email: irina_nt2013@mail.ru
ORCID iD: 0000-0001-9225-5315
SPIN-code: 9184-3509
Russian Federation, 2, Moscowski trakt, Tomsk, 634050
References
- Korenberg EI, Pomelova VG, Osin NS. Infections with natural focality transmitted by ixodid ticks. Moscow; 2013. 464 p. (In Russ).
- Rudakova SA, Pen’evskaya NA, Blokh AI, et al. Review of the epidemiological situation on ixodic tick-borne borreliosis in the Russian Federation in 2010–2020 and prognosis for 2021. Problems of Particularly Dangerous Infections. 2021;(2):52–61. (In Russ). doi: 10.21055/0370-1069-2021-2-52-61
- Platonov AE, Karan’ LS, Kolyasnikova NM, et al. Taxonomic position and genetic diversity of the borrelia species Borrelia miyamotoi ― the causative agent of the “new” ixodic tick-borne borreliosis. Molecular diagnostics – 2010: Proceedings of the All-Russian scientific and practical conference with international participation. Vol. 2. Ed. by V.I. Pokrovsky. Moscow: EhiViDzhi. Р. 250–256. (In Russ).
- Sarksyan DS. Ixodic tick-borne borreliosis ― the current state of the problem. Infectious diseases. 2015;13(2):61–67. (In Russ).
- Platonov AE, Maleev VV, Karan’ LS, et al. Relapsing fever borrelioses: forgotten and new ones. Ther Arch. 2010;82(11):74–80. (In Russ).
- Krasnova EI, Savel’eva MV, Khokhlova NI, et al. Features of clinical manifestations and laboratory diagnostics of recurrent tick fever caused by Borrelia miyamotoi in the Novosibirsk region. Epidemiology and infectious diseases. 2017;(2):10–14. (In Russ).
- Kubiak K, Szczotko M, Dmitryjuk M. Borrelia miyamotoi ― an emerging human tick-borne pathogen in Europe. Microorganisms. 2021;9(1):154. doi: 10.3390/microorganisms9010154
- Cutler SJ. Relapsing fever borreliae: a global review. Clin Lab Med. 2015;35(4):847–865. doi: 10.1016/j.cll.2015.07.001
- Stukolova OA, Kolyasnikova NM, Sarksyan DS, et al. Development and use of a planar protein biochip for the serological diagnostics of tick-borne borreliosis caused by Borrelia miyamotoi. Molecular Diagnostics – 2017: Proceedings of the IX All-Russian scientific and practical conference with International participation, April 18–20, 2017. Ed. by V.I. Pokrovsky. Moscow: Yulis; 2017. Р. 151–152. (In Russ).
- On the state of sanitary and epidemiological welfare of the population in the Tomsk region in 2021. The State report. Tomsk, 2022. 173 p. (In Russ).
- On the state of sanitary and epidemiological welfare of the population in the Russian Federation in 2020. The State report. Moscow; 2021. 256 p. (In Russ).
- On the state of sanitary and epidemiological welfare of the population in the Tomsk region in 2020. The State report. Tomsk, 2021. 187 p. (In Russ).
- Rudakova SA, Teslova OE, Kaneshova NE, et al. Genospecies diversity of borrelia in ixodes ticks of the West Siberia. Problems of Particularly Dangerous Infections. 2019;(4):92–96. (In Russ). doi: 10.21055/0370-1069-2019-4-92-96
- Kolyasnikova NM, Sarkisyan DS, Toporkova MG, et al. Epidemiological and clinical aspects of non-erythemic forms of ixodic tick-borne borreliosis in Russia. MediAl’ J. 2018;2(22):59–60. (In Russ).
- Platonov AE, Karan LS, Kolyasnikova NM, et al. Humans infected with the relapsing fever spirochete Borrelia miyamotoi, Russia. Emerg Infect Dis. 2011;17(10):1816–1822. doi: 10.3201/eid1710.101474
- Khasnatinov MA, Danchinova GA, Takano A, et al. Prevalence of Borrelia miyamotoi in Ixodes persulcatus in Irkutsk city and its neighboring territories, Russia. Ticks Tick Borne Dis. 2016;7(2): 394–397. doi: 10.1016/j.ttbdis.2015.12.016
- Leonova GN, Lubova VA, Ivanis VA. Monitoring of pathogens of tick-borne infections on the territory of Primorsky Krai in 2014–2018. Pacific Medical Journal. 2018;4(74):10–14. (In Russ). doi: 10.17238/PmJ1609-1175.2018.4.10-14
- Alexandrov GO, Karpova MR, Pototskaya YuA, et al. Determination of Borrelia miyamotoi ― the causative agent of tick-borne recurrent fever in the ticks and blood of a patient in the Tomsk region. Molecular Diagnostics – 2017: Proceedings of the IX All-Russian scientific and practical conference with International participation, April 18–20, 2017. Ed. by V.I. Pokrovsky. Moscow: Yulis; 2017. Р. 338–339. (In Russ).
- Kondrashova AM, Karpova MR, Bondarenko EI, et al. The first confirmed case of tick-borne recurrent fever caused by Borrelia miyamotoi in the Tomsk region. Infection and Immunity. 2017;(S):450. (In Russ).
Supplementary files
