Synthesis of silver nanoparticles modified with lysozyme and sodium dioctyl sulfosuccinate and comparison of their biological activities
- Authors: Golubeva O.Y.1, Golubkov V.V.1, Yukhnev V.A.2, Shamova O.V.2,3
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Affiliations:
- Grebenshchikov Institute of Silicate Chemistry
- Institute of Experimantal Medicine
- St. Petersburg State University
- Issue: Vol 43, No 1 (2017)
- Pages: 63-69
- Section: Article
- URL: https://journals.rcsi.science/1087-6596/article/view/215866
- DOI: https://doi.org/10.1134/S1087659617010060
- ID: 215866
Cite item
Abstract
Silver nanoparticles have been fabricated by the method of chemical reduction in solution using different types of stabilizers: an organic low-molecular compound—sodium dioctyl sulfosuccinate (AOT)— and a natural biologically active substance—antimicrobial cationic polypeptide lysozyme. According to studies of the produced hydrosols, the average size of the shell-coated particles is 20–25 nm. The biological activity of the obtained bioconjugates toward Gram-negative (Escherichia coli ML35p, Pseudomonas aeruginosa (clinical isolate)) and Gram-positive (Listeria monocytogenes EGD (АТСС ВАА-679), MRSA ATCC 33591 (Staphylococcus aureus resistant to methicillin)) bacteria has been investigated by the methods of radial diffusion in an agarose gel and serial dilution in a liquid nutritional medium. It has been established that the antimicrobial activity of the bioconjugates depends on the nature of the used stabilizer.
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About the authors
O. Yu. Golubeva
Grebenshchikov Institute of Silicate Chemistry
Author for correspondence.
Email: olga_isc@mail.ru
Russian Federation, St. Petersburg, 199034
V. V. Golubkov
Grebenshchikov Institute of Silicate Chemistry
Email: olga_isc@mail.ru
Russian Federation, St. Petersburg, 199034
V. A. Yukhnev
Institute of Experimantal Medicine
Email: olga_isc@mail.ru
Russian Federation, St. Petersburg, 197376
O. V. Shamova
Institute of Experimantal Medicine; St. Petersburg State University
Email: olga_isc@mail.ru
Russian Federation, St. Petersburg, 197376; St. Petersburg, 199034