Preparation and characterization of temperature response molecularly imprinted membrane with chitosan and methylmethacrylate

Abstract

In this study, a novel temperature controlled molecularly imprinted membrane (Temp-Ctrl-MIM) made of chitosan and methyl methacrylate was fabricated with bovine serum albumin (BSA) as template molecule. The film was characterized by infrared spectroscopy (IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and the permeation experiment. The results revealed that the porous structure of the film was as similar as the BSA. Moreover, the membrane had advanced molecular imprinting capability to BSA comparing to pepsin and casein at any temperature and the film demonstrated an excellent ability to identify specific template molecule (BSA) at the synthesis temperature comparing to other temperatures.