Effect of ligands binding on the isotherm of hybridization of the DNA-chip

H. L. Tsaturyan; Sh. A. Tonoyan; V. F. Morozov; Y. Sh. Mamasakhlisov

doi:10.3103/S106833721702013X

Effect of ligands binding on the isotherm of hybridization of the DNA-chip

Authors: Tsaturyan H.L.¹, Tonoyan S.A.¹, Morozov V.F.¹, Mamasakhlisov Y.S.¹
Affiliations:
1. Yerevan State University
Issue: Vol 52, No 2 (2017)
Pages: 180-188
Section: Article
URL: https://journals.rcsi.science/1068-3372/article/view/228381
DOI: https://doi.org/10.3103/S106833721702013X
ID: 228381

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Abstract

The factors are analyzed, which influence both the hybridization thermodynamics and the DNA/DNA-duplex stability. The non-competitive hybridization of the DNA as well as the competitive hybridization on the surface are investigated. It is shown that the binding to the intercalating ligands results to an increase in the selectivity and sensitivity of the DNA chips.