Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67
- 作者: Arbatsky N.1, Shneider M.2, Shashkov A.1, Popova A.3,4, Miroshnikov K.2, Volozhantsev N.4, Knirel Y.1
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隶属关系:
- N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Moscow Institute of Physics and Technology
- State Research Center for Applied Microbiology and Biotechnology
- 期: 卷 65, 编号 2 (2016)
- 页面: 588-591
- 栏目: Full Articles
- URL: https://journals.rcsi.science/1066-5285/article/view/237390
- DOI: https://doi.org/10.1007/s11172-016-1342-y
- ID: 237390
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详细
Capsular polysaccharide (CPS) was isolated from a nosocomial pathogen Acinetobacter baumannii (A. baumannii) NIPH67 and studied by sugar analysis, Smith degradation, and 1H and 13C NMR spectroscopy. The CPS was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac), and the structure of the linear trisaccharide repeating unit of the CPS was established as →4)-α-Psep5Ac7Ac-(2→6)-β-d-Galp-(1→3)-α-d-GalpNAc-(1→. The genetic content of the capsule biosynthesis cluster of A. baumannii NIPH67, designated KL33, is consistent with the established CPS structure, and thus the capsule of the investigated strain was assigned to K33 group. Functions of proteins including two glycosyltransferases encoded by the genes of the K33 locus were assigned based on the structure of CPS and by the comparison with related proteins of other capsular types of A. baumannii.
作者简介
N. Arbatsky
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: yknirel@gmail.com
俄罗斯联邦, 47 Leninsky prosp., Moscow, 119991
M. Shneider
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yknirel@gmail.com
俄罗斯联邦, 16/10 ul. Miklukho-Maklaya, Moscow, 117997
A. Shashkov
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: yknirel@gmail.com
俄罗斯联邦, 47 Leninsky prosp., Moscow, 119991
A. Popova
Moscow Institute of Physics and Technology; State Research Center for Applied Microbiology and Biotechnology
Email: yknirel@gmail.com
俄罗斯联邦, 9 Institutsky per., Dolgoprudnyi, Moscow Region, 141700; Obolensk, Moscow Region, 142279
K. Miroshnikov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yknirel@gmail.com
俄罗斯联邦, 16/10 ul. Miklukho-Maklaya, Moscow, 117997
N. Volozhantsev
State Research Center for Applied Microbiology and Biotechnology
Email: yknirel@gmail.com
俄罗斯联邦, Obolensk, Moscow Region, 142279
Y. Knirel
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: yknirel@gmail.com
俄罗斯联邦, 47 Leninsky prosp., Moscow, 119991