New DNA-sensor based on thiacalix[4]arene-modified polydiacetylene particles
- Авторы: Valiyakhmetova A.1, Sultanova E.1,2, Burilov V.1, Solovieva S.1,2, Antipin I.1,2
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Учреждения:
- Kazan (Volga Region) Federal University
- A. E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences
- Выпуск: Том 68, № 5 (2019)
- Страницы: 1067-1074
- Раздел: Full Article
- URL: https://journals.rcsi.science/1066-5285/article/view/243382
- DOI: https://doi.org/10.1007/s11172-019-2521-4
- ID: 243382
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Аннотация
New p-tert-butyl thiacalix[4]arene derivative in the 1,3-alternate stereoisomeric form containing two diethylenetriamine groups and pentacosa-10,12-diynoic moieties on the opposide sides of macrocyclic cavity was synthesized using the copper(i)-catalyzed azide-alkyne cycloaddition. According to the dynamic and electrophoretic light scattering data, the synthesized macrocycle forms submicron particles with the sizes 200 nm and ζ-potential equal to 43 mV. The critical aggregation concentration of the macrocycle was 0.019 mmol L−1. The obtained macrocycle intercalates into calf thymus DNA (CT DNA) to form a lipoplex. Using ethidium bromide as a fluorescent probe intercalation of obtained macrocycle into CT DNA with following formation of a lipoplex with the ζ-potential equal to −30 mV was found. The macrocycle was used for the synthesis of mixed polydiacetylene particles with N-(2-aminoethyl)pentacosa-10,12-diynamide (PCDA) as a base lipid. The highest degree of polymerization is achieved in the system with the macrocycle to PCDA ratio equal to 1 : 4. Macrocycle embedding into the polydiacetylene particles significantly increases their colorimetric response to CT DNA. The response to CT DNA as a change in the color of a solution of particles from blue to red is seen by naked eye at the CT DNA concentration starting from 20 µmol L−1, which makes the obtained particles promising for bioanalytical application.
Об авторах
A. Valiyakhmetova
Kazan (Volga Region) Federal University
Email: ultrav@bk.ru
Россия, 18 ul. Kremlevskaya, Kazan, 420008
E. Sultanova
Kazan (Volga Region) Federal University; A. E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences
Email: ultrav@bk.ru
Россия, 18 ul. Kremlevskaya, Kazan, 420008; 8 ul. Akad. Arbuzova, Kazan, 420008
V. Burilov
Kazan (Volga Region) Federal University
Автор, ответственный за переписку.
Email: ultrav@bk.ru
Россия, 18 ul. Kremlevskaya, Kazan, 420008
S. Solovieva
Kazan (Volga Region) Federal University; A. E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences
Email: ultrav@bk.ru
Россия, 18 ul. Kremlevskaya, Kazan, 420008; 8 ul. Akad. Arbuzova, Kazan, 420008
I. Antipin
Kazan (Volga Region) Federal University; A. E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences
Email: ultrav@bk.ru
Россия, 18 ul. Kremlevskaya, Kazan, 420008; 8 ul. Akad. Arbuzova, Kazan, 420008