Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles
- Авторлар: Demin A.1, Valova M.1, Pershina A.2,3, Krasnov V.1
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Мекемелер:
- I. Ya. Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences
- Siberian State Medical University
- National Research Tomsk Polytechnic University
- Шығарылым: Том 68, № 5 (2019)
- Беттер: 1088-1095
- Бөлім: Full Article
- URL: https://journals.rcsi.science/1066-5285/article/view/243385
- DOI: https://doi.org/10.1007/s11172-019-2524-1
- ID: 243385
Дәйексөз келтіру
Аннотация
The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional groups, the medium, and the presence of a coupling agent, namely, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), was studied using fluorimetry, UV and IR spectroscopy. It was shown that protein immobilization can occur by both non-covalent and covalent binding with surface groups of MNPs. The best results (up to 150 μg of TagGFP2 per 1 mg of MNPs) were obtained for MNPs preliminarily functionalized with PMIDA and using EDC. The results of this study can be used for the design of imaging agents having both magnetic and optical properties to be applied in biomedicine.
Авторлар туралы
A. Demin
I. Ya. Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: demin@ios.uran.ru
Ресей, 22 ul. S. Kovalevskoi, Ekaterinburg, 620137
M. Valova
I. Ya. Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences
Email: demin@ios.uran.ru
Ресей, 22 ul. S. Kovalevskoi, Ekaterinburg, 620137
A. Pershina
Siberian State Medical University; National Research Tomsk Polytechnic University
Email: demin@ios.uran.ru
Ресей, 2/18 Moskovsky trakt, Tomsk, 634050; 30 prosp. Lenina, Tomsk, 634050
V. Krasnov
I. Ya. Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences
Email: demin@ios.uran.ru
Ресей, 22 ul. S. Kovalevskoi, Ekaterinburg, 620137