Structure of the O-polysaccharide of Yersinia frederiksenii H56-36/81 (serotype O:60) containing 4-deoxy-d-arabino-hexose
- Authors: Sizova O.V.1, Shashkov A.S.1, Shaikhutdinova R.Z.2, Ivanov S.A.2, Dentovskaya S.V.2, Knirel Y.A.1
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Affiliations:
- N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
- State Research Center for Applied Microbiology and Biotechnology
- Issue: Vol 65, No 6 (2016)
- Pages: 1625-1629
- Section: Full Articles
- URL: https://journals.rcsi.science/1066-5285/article/view/238474
- DOI: https://doi.org/10.1007/s11172-016-1490-0
- ID: 238474
Cite item
Abstract
Aiming at creation of the chemical basis for serotyping of strains of Yersinia spp., we studied the O-specific polysaccharide (OPS) of Yersinia frederiksenii H56-36/81 belonging to serotype O:60. A branched polysaccharide was obtained by mild alkaline degradation of the lipopolysaccharide (LPS) isolated from bacterial cells by the phenol—water extraction and found to contain d-ribose, l-rhamnose, and 4-deoxy d-arabino-hexose (d-ara4dHex). The last monosaccharide occurs rarely in nature and earlier has been found only in OPS of Citrobacter spp. Mild acid hydrolysis of the LPS resulted in cleavage of the d-ara4dHex side-chains to give a linear polysaccharide. Structure of the hexasaccharide repeating unit of the OPS was established by studies of both isolated polysaccharides by 1D and 2D 1H and 13C NMR spectroscopy.
About the authors
O. V. Sizova
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: izvan@ioc.ac.ru
Russian Federation, 47 Leninsky prosp., Moscow, 119991
A. S. Shashkov
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: izvan@ioc.ac.ru
Russian Federation, 47 Leninsky prosp., Moscow, 119991
R. Z. Shaikhutdinova
State Research Center for Applied Microbiology and Biotechnology
Email: izvan@ioc.ac.ru
Russian Federation, Obolensk, Moscow Region, 142279
S. A. Ivanov
State Research Center for Applied Microbiology and Biotechnology
Email: izvan@ioc.ac.ru
Russian Federation, Obolensk, Moscow Region, 142279
S. V. Dentovskaya
State Research Center for Applied Microbiology and Biotechnology
Email: izvan@ioc.ac.ru
Russian Federation, Obolensk, Moscow Region, 142279
Yu. A. Knirel
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: izvan@ioc.ac.ru
Russian Federation, 47 Leninsky prosp., Moscow, 119991