Radioimmunochemical studies for development of a solid-phase radioimmunoassay system using coated beads for the assessment of human chorionic gonadotropin
- 作者: Sallam K.1, El-Bayoumy A.1, Mehany N.1
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隶属关系:
- Labeled Compounds Department, Hot Laboratories Center
- 期: 卷 59, 编号 2 (2017)
- 页面: 181-187
- 栏目: Article
- URL: https://journals.rcsi.science/1066-3622/article/view/223832
- DOI: https://doi.org/10.1134/S1066362217020126
- ID: 223832
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详细
A method was developed for immobilization of prepared human chorionic gonadotropin (β-hCG) antisera on fresh plastic beads through a covalent immune bridge. Such covalent immune-coated antisera on plastic beads were used in the development of a noncentrifugation radioimmunoassay. 125I-β-hCG was prepared using different oxidizing agents. The solid-phase RIA system developed was found to well compete with the conventional method.
作者简介
Kh. Sallam
Labeled Compounds Department, Hot Laboratories Center
Email: aasami72@gmail.com
埃及, Cairo
A. El-Bayoumy
Labeled Compounds Department, Hot Laboratories Center
编辑信件的主要联系方式.
Email: aasami72@gmail.com
埃及, Cairo
N. Mehany
Labeled Compounds Department, Hot Laboratories Center
Email: aasami72@gmail.com
埃及, Cairo