Email this article Radioimmunochemical studies for development of a solid-phase radioimmunoassay system using coated beads for the assessment of human chorionic gonadotropin
- Authors: Sallam K.M.1, El-Bayoumy A.S.1, Mehany N.L.1
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Affiliations:
- Labeled Compounds Department, Hot Laboratories Center
- Issue: Vol 59, No 2 (2017)
- Pages: 181-187
- Section: Article
- URL: https://journals.rcsi.science/1066-3622/article/view/223832
- DOI: https://doi.org/10.1134/S1066362217020126
- ID: 223832
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Abstract
A method was developed for immobilization of prepared human chorionic gonadotropin (β-hCG) antisera on fresh plastic beads through a covalent immune bridge. Such covalent immune-coated antisera on plastic beads were used in the development of a noncentrifugation radioimmunoassay. 125I-β-hCG was prepared using different oxidizing agents. The solid-phase RIA system developed was found to well compete with the conventional method.
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About the authors
Kh. M. Sallam
Labeled Compounds Department, Hot Laboratories Center
Email: aasami72@gmail.com
Egypt, Cairo
A. S. A. El-Bayoumy
Labeled Compounds Department, Hot Laboratories Center
Author for correspondence.
Email: aasami72@gmail.com
Egypt, Cairo
N. L. Mehany
Labeled Compounds Department, Hot Laboratories Center
Email: aasami72@gmail.com
Egypt, Cairo
