Distribution of Metabolically Active Prokaryotes (Archaea and Bacteria) throughout the Profiles of Chernozem and Brown Semidesert Soil
- Autores: Semenov M.1,2, Manucharova N.1, Stepanov A.1
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Afiliações:
- Faculty of Soil Science
- Dokuchaev Soil Science Institute
- Edição: Volume 49, Nº 2 (2016)
- Páginas: 217-225
- Seção: Soil Biology
- URL: https://journals.rcsi.science/1064-2293/article/view/223700
- DOI: https://doi.org/10.1134/S1064229316020101
- ID: 223700
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Resumo
The distribution of metabolically active cells of archaea and bacteria in the profiles of typical chernozems (Voronezh oblast) and brown semidesert soils (Astrakhan oblast) of natural and agricultural ecosystems was studied using the method of fluorescent in situ hybridization (FISH). The studied soils differed sharply in the microbial biomass and in the numbers of metabolically active cells of archaea and bacteria. The number of active bacterial cells was 3.5–7.0 times greater than that of archaea. In the arable chernozem, the numbers of active cells of archaea and bacteria were 2.6 and 1.5 times, respectively, lower than those in the chernozem under the shelterbelt. The agricultural use of the brown semidesert soil had little effect on the abundances of bacteria and archaea. The soil organic carbon content was the major factor controlling the numbers of metabolically active cells of both domains. However, the dependence of the abundance of bacteria on the organic matter content was more pronounced. The decrease in the organic carbon and total nitrogen contents down the soil profiles was accompanied by the decrease in the bacteria: archaea ratio attesting to a better adaptation of archaea to the permanent deficiency of carbon and nitrogen. The bacteria: archaea ratio can serve as an ecotrophic indicator of the state of soil microbial communities.
Sobre autores
M. Semenov
Faculty of Soil Science; Dokuchaev Soil Science Institute
Autor responsável pela correspondência
Email: mikhail.v.semenov@gmail.com
Rússia, Moscow, 119991; per. Pyzhevskii 7, Moscow, 119017
N. Manucharova
Faculty of Soil Science
Email: mikhail.v.semenov@gmail.com
Rússia, Moscow, 119991
A. Stepanov
Faculty of Soil Science
Email: mikhail.v.semenov@gmail.com
Rússia, Moscow, 119991