Enzymatic activity inside and outside of water-stable aggregates in soils under different land use
- Authors: Garbuz S.A.1, Yaroslavtseva N.V.1, Kholodov V.A.1
-
Affiliations:
- Dokuchaev Soil Science Institute
- Issue: Vol 49, No 3 (2016)
- Pages: 367-375
- Section: Soil Biology
- URL: https://journals.rcsi.science/1064-2293/article/view/223789
- DOI: https://doi.org/10.1134/S1064229316030030
- ID: 223789
Cite item
Abstract
A method is presented for assessing the distribution of enzymatic activity inside and outside of water-stable aggregates. Two samples of water-stable aggregates >1 mm have been isolated from dry aggregates of 1–2 mm. To determine the enzymatic activity, a substrate has been added to one of the samples without disaggregation; the other sample has been preliminarily disaggregated. Enzymatic activity within waterstable aggregates has been assessed from the difference between the obtained results under the supposition that the penetration of substrate within the water-saturated aggregates is hampered, and enzymatic reactions occur only at the periphery. The levels and distributions of enzymatic (peroxidase, polyphenol oxidase, and catalase) activities in water-stable aggregates of soddy-podzolic soils under forest and plowland and typical chernozems of long-term field experiments have been studied. The peroxidase, polyphenol oxidase, and catalase activities of water-stable aggregates vary from 6 to 23, from 7 to 30, and from 5 to 7 mmol/(g h), respectively. The ratio between the enzymatic activities inside and outside of soil aggregates showed a higher dependence on soil type and land use, as well as on the input of organic matter and the structural state, than the general activity level in water-stable aggregates.
About the authors
S. A. Garbuz
Dokuchaev Soil Science Institute
Author for correspondence.
Email: st_garbuz@mail.ru
Russian Federation, Pyzhevskii per. 7, Moscow, 119017
N. V. Yaroslavtseva
Dokuchaev Soil Science Institute
Email: st_garbuz@mail.ru
Russian Federation, Pyzhevskii per. 7, Moscow, 119017
V. A. Kholodov
Dokuchaev Soil Science Institute
Email: st_garbuz@mail.ru
Russian Federation, Pyzhevskii per. 7, Moscow, 119017