Polarized Fluorescence in NADH Two-Photon Excited by Femtosecond Laser Pulses in the Wavelength Range of 720–780 nm

M. E. Sasin; I. A. Gorbunova; N. O. Bezverkhnii; Y. M. Beltukov; O. S. Vasyutinskii; J. Rubayo-Soneira

doi:10.1134/S1063785019070101

Polarized Fluorescence in NADH Two-Photon Excited by Femtosecond Laser Pulses in the Wavelength Range of 720–780 nm

Authors: Sasin M.E.¹, Gorbunova I.A.¹, Bezverkhnii N.O.¹, Beltukov Y.M.¹, Vasyutinskii O.S.¹, Rubayo-Soneira J.²
Affiliations:
1. Ioffe Physical Technical Institute, Russian Academy of Sciences
2. Universidad de La Habana
Issue: Vol 45, No 7 (2019)
Pages: 672-674
Section: Article
URL: https://journals.rcsi.science/1063-7850/article/view/208362
DOI: https://doi.org/10.1134/S1063785019070101
ID: 208362

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Abstract

The decay of polarized fluorescence in coenzyme NADH in aqueous-methanol solution under two-photon excitation by femtosecond laser pulses within the spectral range of 720–780 nm has been studied. Molecular fluorescence parameters: decay times τ₁ and τ₂, rotational diffusion time τ_rot, and pre-exponential factor ratio a₂/a₁ have been determined from experiment. The results obtained have been compared with those reported recently in NADH in aqueous solution.