Thermodynamic Analysis of the Conformational Stability of a Single-Domain Therapeutic Antibody
- 作者: Eliseev I.E.1, Yudenko A.N.1, Besedina N.A.1, Ulitin A.B.2, Ekimova V.M.2, Evdokimov S.R.2, Putintceva J.V.2, Yakovlev P.A.2, Lomovskaya M.I.2, Terterov I.N.1, Bogdanov A.A.1, Dubina M.V.3
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隶属关系:
- St. Petersburg National Research Academic University
- CJSC Biocad
- Pavlov First St. Petersburg State Medical University
- 期: 卷 43, 编号 12 (2017)
- 页面: 1088-1091
- 栏目: Article
- URL: https://journals.rcsi.science/1063-7850/article/view/206774
- DOI: https://doi.org/10.1134/S1063785017120045
- ID: 206774
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详细
The stability of a new single-domain therapeutic antibody to the ErbB3 receptor was studied by fluorescence spectroscopy at different concentrations of a denaturing agent and temperatures. The analysis of experimental denaturation curves allowed us to build a complete thermodynamic model of unfolding and to determine all parameters of the transition: ΔG = 8.5 kcal mol–1, Tm = 76°C, ΔHm = 107 kcal mol–1, ΔCp = 1.8 kcal K–1 mol–1. The obtained data evidence the high stability of the antibody in a broad range of conditions, which is essential for further structural and functional studies and possible therapeutic application.
作者简介
I. Eliseev
St. Petersburg National Research Academic University
编辑信件的主要联系方式.
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021
A. Yudenko
St. Petersburg National Research Academic University
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021
N. Besedina
St. Petersburg National Research Academic University
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021
A. Ulitin
CJSC Biocad
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515
V. Ekimova
CJSC Biocad
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515
S. Evdokimov
CJSC Biocad
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515
J. Putintceva
CJSC Biocad
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515
P. Yakovlev
CJSC Biocad
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515
M. Lomovskaya
CJSC Biocad
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515
I. Terterov
St. Petersburg National Research Academic University
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021
A. Bogdanov
St. Petersburg National Research Academic University
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021
M. Dubina
Pavlov First St. Petersburg State Medical University
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 197022
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