Thermodynamic Analysis of the Conformational Stability of a Single-Domain Therapeutic Antibody


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The stability of a new single-domain therapeutic antibody to the ErbB3 receptor was studied by fluorescence spectroscopy at different concentrations of a denaturing agent and temperatures. The analysis of experimental denaturation curves allowed us to build a complete thermodynamic model of unfolding and to determine all parameters of the transition: ΔG = 8.5 kcal mol–1, Tm = 76°C, ΔHm = 107 kcal mol–1, ΔCp = 1.8 kcal K–1 mol–1. The obtained data evidence the high stability of the antibody in a broad range of conditions, which is essential for further structural and functional studies and possible therapeutic application.

作者简介

I. Eliseev

St. Petersburg National Research Academic University

编辑信件的主要联系方式.
Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021

A. Yudenko

St. Petersburg National Research Academic University

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021

N. Besedina

St. Petersburg National Research Academic University

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021

A. Ulitin

CJSC Biocad

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515

V. Ekimova

CJSC Biocad

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515

S. Evdokimov

CJSC Biocad

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515

J. Putintceva

CJSC Biocad

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515

P. Yakovlev

CJSC Biocad

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515

M. Lomovskaya

CJSC Biocad

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 198515

I. Terterov

St. Petersburg National Research Academic University

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021

A. Bogdanov

St. Petersburg National Research Academic University

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 194021

M. Dubina

Pavlov First St. Petersburg State Medical University

Email: eliseev@spbau.ru
俄罗斯联邦, St. Petersburg, 197022

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