Simulation of Kinetic Curves of Polymerase Chain Reaction Obtained Using Fluorescent Oligonucleotide Probes
- Authors: Fedorov A.A.1, Sochivko D.G.2, Varlamov D.A.3, Kurochkin V.E.1
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Affiliations:
- Institute for Analytical Instrumentation, Russian Academy of Sciences
- OOO Sintol
- All-Russia Research Institute of Agricultural Biotechnology
- Issue: Vol 63, No 10 (2018)
- Pages: 1430-1434
- Section: Instrument Development and Devices for Practical Applications
- URL: https://journals.rcsi.science/1063-7842/article/view/202097
- DOI: https://doi.org/10.1134/S1063784218100109
- ID: 202097
Cite item
Abstract
At present, many polymerase chain reaction models have been proposed for exact quantitative estimation of the reaction results. In most models, kinetics of product accumulation and kinetics of fluorescent reporter are assumed to be identical. A model of the polymerase chain reaction is proposed to study the difference of such functions in the system in which a hybridization probe serves as the source of the detected fluorescence signal. Adequacy of the model is verified, and significant possible difference of the accumulation kinetics of product and probe reporter is demonstrated.
About the authors
A. A. Fedorov
Institute for Analytical Instrumentation, Russian Academy of Sciences
Author for correspondence.
Email: f_aa@mail.ru
Russian Federation, St. Petersburg, 190103
D. G. Sochivko
OOO Sintol
Email: f_aa@mail.ru
Russian Federation, Moscow, 127550
D. A. Varlamov
All-Russia Research Institute of Agricultural Biotechnology
Email: f_aa@mail.ru
Russian Federation, Moscow, 127550
V. E. Kurochkin
Institute for Analytical Instrumentation, Russian Academy of Sciences
Email: f_aa@mail.ru
Russian Federation, St. Petersburg, 190103