The Single Cells and Cell Populations Viability Estimation in vitro by the Time-Domain Impedance Spectroscopy
- Authors: Stupin D.D.1,2
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Affiliations:
- St. Petersburg Academic University
- ITMO University
- Issue: Vol 63, No 9 (2018)
- Pages: 1384-1389
- Section: Instrument Development and Devices for Practical Applications
- URL: https://journals.rcsi.science/1063-7842/article/view/202046
- DOI: https://doi.org/10.1134/S1063784218090219
- ID: 202046
Cite item
Abstract
In the present study, we investigate the sensitivity and applicability of the time-domain electrical impedance spectroscopy (EIS) techniques, namely Fourier-EIS and adaptive filtering based EIS (AF-EIS), for studying cells' populations and single living cells in natural physiological environment in vitro. Using ultra-violet radiation for decreasing cell life-span we demonstrate the possibility to distinguish the living cells' population from the dead cells by both Fourier-EIS and AF-EIS. However, determining the viability of the single cell requires significant sensitivity, which is inaccessible with the conventional Fourier-EIS contrary to AF-EIS. The latter result stems from the high noise immunity of the AF-EIS, which also makes it possible to provide the measurements using the safe for cells 15-mV excitation voltage and 10–100 nA current response. The developed single-cell AF-EIS approach opens a direct roadmap for creating accurate, robust, and easy to implement toxin and radiation hazard sensors with the living cell as an acting element and proposes a solution for actual ecological and healthcare problems.
About the authors
D. D. Stupin
St. Petersburg Academic University; ITMO University
Author for correspondence.
Email: Stu87@ya.ru
Russian Federation, St. Petersburg, 194021; St. Petersburg, 197101