Development of a Microfluidic Chip for Protein Crystallization by the Microbatch Method
- 作者: Popov A.1, Dorovatovskii P.1, Mamichev D.1, Marchenkova M.1,2, Nikolaeva A.1
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隶属关系:
- National Research Centre “Kurchatov Institute”
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
- 期: 卷 64, 编号 2 (2019)
- 页面: 282-286
- 栏目: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/193825
- DOI: https://doi.org/10.1134/S1063774519020226
- ID: 193825
如何引用文章
详细
A composite microfluidic chip (MFC), which comprises a technological system for protein crystal growth followed by X-ray crystallography, was developed. Crystallization tests were performed using chicken egg-white lysozyme, aminotransferase from Methanococcus vannielii, and esterase PMGL2 from the permafrost metagenomic library. Materials (borosilicate glass and poly(methyl methacrylate)) for MFC fabrication were compared. The use of poly(methyl methacrylate) was shown to allow comparative in situ X-ray crystallography of protein crystals directly in the chip. Investigations and experiments were carried out at the BELOK beamline at the Kurchatov synchrotron radiation source (National Research Centre “Kurchatov Institute”).
作者简介
A. Popov
National Research Centre “Kurchatov Institute”
编辑信件的主要联系方式.
Email: antmp.86@yandex.ru
俄罗斯联邦, Moscow, 123098
P. Dorovatovskii
National Research Centre “Kurchatov Institute”
Email: antmp.86@yandex.ru
俄罗斯联邦, Moscow, 123098
D. Mamichev
National Research Centre “Kurchatov Institute”
Email: antmp.86@yandex.ru
俄罗斯联邦, Moscow, 123098
M. Marchenkova
National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences
Email: antmp.86@yandex.ru
俄罗斯联邦, Moscow, 123098; Moscow, 119333
A. Nikolaeva
National Research Centre “Kurchatov Institute”
Email: antmp.86@yandex.ru
俄罗斯联邦, Moscow, 123098