Nature of impurities during protein crystallization
- Авторы: Baskakova S.S.1, Volkov V.V.1, Laptinskaya T.V.2, Lyasnikova M.S.1, Voloshin A.E.1, Koval’chuk M.V.1,3
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Учреждения:
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
- Moscow State University
- National Research Centre “Kurchatov Institute,”
- Выпуск: Том 62, № 1 (2017)
- Страницы: 148-156
- Раздел: Crystal Growth
- URL: https://journals.rcsi.science/1063-7745/article/view/190665
- DOI: https://doi.org/10.1134/S1063774517010060
- ID: 190665
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Аннотация
Lysozyme crystal growth was studied using reagents of different purity of three trademarks— Seikagaku Corporation (sixfold recrystallized lysozyme), Sigma-Aldrich (threefold recrystallized lysozyme), and Hampton Research (threefold recrystallized lysozyme). Solutions of these reagents were investigated by small-angle X-ray scattering, dynamic light scattering (DLS), ultracentrifugation, and electrophoresis. It was found that crystal-growth and oligomerization processes are more intense in solutions of the reagent of higher purity. The dependences of the fraction of lysozyme oligomers on the supersaturation and purity of the solution are analyzed.
Об авторах
S. Baskakova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
Автор, ответственный за переписку.
Email: SvetlBaskakova@yandex.ru
Россия, Moscow, 119333
V. Volkov
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
Email: SvetlBaskakova@yandex.ru
Россия, Moscow, 119333
T. Laptinskaya
Moscow State University
Email: SvetlBaskakova@yandex.ru
Россия, Moscow, 119992
M. Lyasnikova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
Email: SvetlBaskakova@yandex.ru
Россия, Moscow, 119333
A. Voloshin
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
Email: SvetlBaskakova@yandex.ru
Россия, Moscow, 119333
M. Koval’chuk
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute,”
Email: SvetlBaskakova@yandex.ru
Россия, Moscow, 119333; Moscow, 123098
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