Development of a Microfluidic Chip for Protein Crystallization by the Microbatch Method
- Autores: Popov A.1, Dorovatovskii P.1, Mamichev D.1, Marchenkova M.1,2, Nikolaeva A.1
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Afiliações:
- National Research Centre “Kurchatov Institute”
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
- Edição: Volume 64, Nº 2 (2019)
- Páginas: 282-286
- Seção: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/193825
- DOI: https://doi.org/10.1134/S1063774519020226
- ID: 193825
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Resumo
A composite microfluidic chip (MFC), which comprises a technological system for protein crystal growth followed by X-ray crystallography, was developed. Crystallization tests were performed using chicken egg-white lysozyme, aminotransferase from Methanococcus vannielii, and esterase PMGL2 from the permafrost metagenomic library. Materials (borosilicate glass and poly(methyl methacrylate)) for MFC fabrication were compared. The use of poly(methyl methacrylate) was shown to allow comparative in situ X-ray crystallography of protein crystals directly in the chip. Investigations and experiments were carried out at the BELOK beamline at the Kurchatov synchrotron radiation source (National Research Centre “Kurchatov Institute”).
Sobre autores
A. Popov
National Research Centre “Kurchatov Institute”
Autor responsável pela correspondência
Email: antmp.86@yandex.ru
Rússia, Moscow, 123098
P. Dorovatovskii
National Research Centre “Kurchatov Institute”
Email: antmp.86@yandex.ru
Rússia, Moscow, 123098
D. Mamichev
National Research Centre “Kurchatov Institute”
Email: antmp.86@yandex.ru
Rússia, Moscow, 123098
M. Marchenkova
National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences
Email: antmp.86@yandex.ru
Rússia, Moscow, 123098; Moscow, 119333
A. Nikolaeva
National Research Centre “Kurchatov Institute”
Email: antmp.86@yandex.ru
Rússia, Moscow, 123098