Small-angle X-Ray analysis of macromolecular structure: the structure of protein NS2 (NEP) in solution


Citar

Texto integral

Acesso aberto Acesso aberto
Acesso é fechado Acesso está concedido
Acesso é fechado Somente assinantes

Resumo

A complex structural analysis of nuclear export protein NS2 (NEP) of influenza virus A has been performed using bioinformatics predictive methods and small-angle X-ray scattering data. The behavior of NEP molecules in a solution (their aggregation, oligomerization, and dissociation, depending on the buffer composition) has been investigated. It was shown that stable associates are formed even in a conventional aqueous salt solution at physiological рН value. For the first time we have managed to get NEP dimers in solution, to analyze their structure, and to compare the models obtained using the method of the molecular tectonics with the spatial protein structure predicted by us using the bioinformatics methods. The results of the study provide a new insight into the structural features of nuclear export protein NS2 (NEP) of the influenza virus A, which is very important for viral infection development.

Sobre autores

E. Shtykova

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”; Semenov Institute of Chemical Physics

Autor responsável pela correspondência
Email: shtykova@ns.crys.ras.ru
Rússia, Moscow, 119333; Moscow, 119991

E. Bogacheva

Semenov Institute of Chemical Physics

Email: shtykova@ns.crys.ras.ru
Rússia, Moscow, 119991

L. Dadinova

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”

Email: shtykova@ns.crys.ras.ru
Rússia, Moscow, 119333

C. Jeffries

EMBL, Hamburg Outstation

Email: shtykova@ns.crys.ras.ru
Alemanha, Hamburg

N. Fedorova

Belozersky Institute of Physico-Chemical Biology

Email: shtykova@ns.crys.ras.ru
Rússia, Moscow

A. Golovko

Faculty of Bioengineering and Bioinformatics

Email: shtykova@ns.crys.ras.ru
Rússia, Moscow

L. Baratova

Belozersky Institute of Physico-Chemical Biology

Email: shtykova@ns.crys.ras.ru
Rússia, Moscow

O. Batishchev

Frumkin Institute of Electrochemistry

Email: shtykova@ns.crys.ras.ru
Rússia, Moscow, 119071

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML

Declaração de direitos autorais © Pleiades Publishing, Inc., 2017