Мақаланы E-mail арқылы жіберу Small-angle X-ray scattering study of the influence of solvent replacement (from H2O to D2O) on the initial crystallization stage of tetragonal lysozyme
- Авторлар: Boikova A.S.1,2, D’yakova Y.A.1,2, Il’ina K.B.1,2, Konarev P.V.1,2, Kryukova A.E.1,2, Marchenkova M.A.1,2, Blagov A.E.1,2, Pisarevskii Y.V.1,2, Koval’chuk M.V.1,2,3
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Мекемелер:
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
- National Research Centre “Kurchatov Institute,”
- St. Petersburg State University
- Шығарылым: Том 62, № 6 (2017)
- Беттер: 837-842
- Бөлім: Diffraction and Scattering of Ionizing Radiations
- URL: https://journals.rcsi.science/1063-7745/article/view/191407
- DOI: https://doi.org/10.1134/S1063774517060074
- ID: 191407
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
The composition of lysozyme solutions in D2O under conditions favorable for the formation of tetragonal crystals has been investigated at different protein concentrations by small-angle X-ray scattering using the synchrotron radiation. In addition to lysozyme monomers, dimeric and octameric species are found in the crystallization solutions; the octamer content increases with an increase in the protein concentration. A comparison of the data with those obtained under similar conditions but with H2O used as a solvent has shown that the replacement of light water with heavy one leads to increase of octamer volume fraction in solution.
Авторлар туралы
A. Boikova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Хат алмасуға жауапты Автор.
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
Yu. D’yakova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
K. Il’ina
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
P. Konarev
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
A. Kryukova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
M. Marchenkova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
A. Blagov
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
Yu. Pisarevskii
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
M. Koval’chuk
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”; St. Petersburg State University
Email: boikova_as@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182; St. Petersburg, 199034
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