Shape Determination of Bovine Fibrinogen in Solution Using Small-Angle Scattering Data

A. E. Kryukova; A. I. Shpichka; P. V. Konarev; V. V. Volkov; P. S. Timashev; V. E. Asadchikov

doi:10.1134/S1063774518060202

Shape Determination of Bovine Fibrinogen in Solution Using Small-Angle Scattering Data

Authors: Kryukova A.E.¹^,2, Shpichka A.I.³, Konarev P.V.¹^,2, Volkov V.V.¹, Timashev P.S.³^,4^,5, Asadchikov V.E.¹
Affiliations:
1. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
2. National Research Centre “Kurchatov Institute,”
3. Institute for Regenerative Medicine, Sechenov University
4. Institute of Photonic Technologies, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
5. Semenov Institute of Chemical Physics, Russian Academy of Sciences
Issue: Vol 63, No 6 (2018)
Pages: 871-873
Section: Diffraction and Scattering of Ionizing Radiations
URL: https://journals.rcsi.science/1063-7745/article/view/193071
DOI: https://doi.org/10.1134/S1063774518060202
ID: 193071

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Abstract

A 3D model of the shape of bovine fibrinogen, one of the proteins responsible for blood coagulation, is constructed based on small-angle X-ray scattering (SAXS) data on bovine fibrinogen in solution, and the oligomeric composition of fibrinogen in solution is estimated. It is shown that fibrinogen molecules form elongated vermicular structures with a length on the order of 50 nm and an effective cross section of about 10 nm. The volume of these particles correlates well with the volume of two fibrinogen dimers. The results obtained may be helpful when applying fibrinogen and its modifications in tissue engineering.

Keywords

Bovine Fibrinogen, Fibrinogen Molecule, Small-angle X-ray Scattering (SAXS), Fibrinogen Dimer, GNOM Program

About the authors

A. E. Kryukova

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”
Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”

Email: asad@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182

A. I. Shpichka

Institute for Regenerative Medicine, Sechenov University

Email: asad@crys.ras.ru
Russian Federation, Moscow, 119991

P. V. Konarev

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”
Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”

Email: asad@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182

V. V. Volkov

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”
Russian Academy of Sciences

Email: asad@crys.ras.ru
Russian Federation, Moscow, 119333

P. S. Timashev

Institute for Regenerative Medicine, Sechenov University; Institute of Photonic Technologies, Federal Scientific Research Centre “Crystallography and Photonics,”
Russian Academy of Sciences; Semenov Institute of Chemical Physics, Russian Academy of Sciences

Email: asad@crys.ras.ru
Russian Federation, Moscow, 119991; Troitsk, 108840; Moscow, 119991

V. E. Asadchikov

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”
Russian Academy of Sciences

Author for correspondence.
Email: asad@crys.ras.ru
Russian Federation, Moscow, 119333

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