Email this article Shape Determination of Bovine Fibrinogen in Solution Using Small-Angle Scattering Data
- Authors: Kryukova A.E.1,2, Shpichka A.I.3, Konarev P.V.1,2, Volkov V.V.1, Timashev P.S.3,4,5, Asadchikov V.E.1
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Affiliations:
- Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
- National Research Centre “Kurchatov Institute,”
- Institute for Regenerative Medicine, Sechenov University
- Institute of Photonic Technologies, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
- Semenov Institute of Chemical Physics, Russian Academy of Sciences
- Issue: Vol 63, No 6 (2018)
- Pages: 871-873
- Section: Diffraction and Scattering of Ionizing Radiations
- URL: https://journals.rcsi.science/1063-7745/article/view/193071
- DOI: https://doi.org/10.1134/S1063774518060202
- ID: 193071
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Abstract
A 3D model of the shape of bovine fibrinogen, one of the proteins responsible for blood coagulation, is constructed based on small-angle X-ray scattering (SAXS) data on bovine fibrinogen in solution, and the oligomeric composition of fibrinogen in solution is estimated. It is shown that fibrinogen molecules form elongated vermicular structures with a length on the order of 50 nm and an effective cross section of about 10 nm. The volume of these particles correlates well with the volume of two fibrinogen dimers. The results obtained may be helpful when applying fibrinogen and its modifications in tissue engineering.
About the authors
A. E. Kryukova
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: asad@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
A. I. Shpichka
Institute for Regenerative Medicine, Sechenov University
Email: asad@crys.ras.ru
Russian Federation, Moscow, 119991
P. V. Konarev
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: asad@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
V. V. Volkov
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences
Email: asad@crys.ras.ru
Russian Federation, Moscow, 119333
P. S. Timashev
Institute for Regenerative Medicine, Sechenov University; Institute of Photonic Technologies, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: asad@crys.ras.ru
Russian Federation, Moscow, 119991; Troitsk, 108840; Moscow, 119991
V. E. Asadchikov
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences
Author for correspondence.
Email: asad@crys.ras.ru
Russian Federation, Moscow, 119333
