Email this article Investigation of the Pre-crystallization Stage of Proteinase K in Solution (Influence of Temperature and Precipitant Type) by Small-Angle X-Ray Scattering
- Authors: Kryukova A.E.1,2, Marchenkova M.A.1,2, Pisarevskii Y.V.1,2, Koval’chuk M.V.1,2,3, Boikova A.S.1,2, D’yakova Y.A.1,2, Il’ina K.B.1,2, Konarev P.V.1,2
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Affiliations:
- Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
- National Research Centre “Kurchatov Institute,”
- St. Petersburg State University
- Issue: Vol 63, No 6 (2018)
- Pages: 865-870
- Section: Diffraction and Scattering of Ionizing Radiations
- URL: https://journals.rcsi.science/1063-7745/article/view/193066
- DOI: https://doi.org/10.1134/S1063774518060068
- ID: 193066
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Abstract
Proteinase K solutions have been investigated by small-angle X-ray scattering in the temperature range of 10–30°C under conditions of tetragonal crystal growth. Addition of ammonium sulfate or sodium nitrate as precipitants is shown to induce the formation of a significant fraction of protein dimers in solution (the dimer content in solution increases only slightly with a decrease in temperature). The structures of possible dimers, which form the pre-crystallization phase in solution (which is necessary for the growth of proteinase K crystals), have been investigated by analyzing the known crystal structure.
About the authors
A. E. Kryukova
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
M. A. Marchenkova
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
Yu. V. Pisarevskii
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
M. V. Koval’chuk
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”; St. Petersburg State University
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182; St. Petersburg, 199034
A. S. Boikova
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Author for correspondence.
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
Yu. A. D’yakova
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
K. B. Il’ina
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
P. V. Konarev
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
