Email this article Temperature Control of Protein Crystal Nucleation
- Authors: Safronov V.V.1, Krivonogova N.V.1, Strelov V.I.1
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Affiliations:
- Space Materials Science Laboratory, Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Kaluga Branch
- Issue: Vol 63, No 2 (2018)
- Pages: 287-289
- Section: Crystal Growth
- URL: https://journals.rcsi.science/1063-7745/article/view/192389
- DOI: https://doi.org/10.1134/S1063774518020232
- ID: 192389
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Abstract
A technique of temperature-controlled biocrystal nucleation, at which the growth onset of one crystal suppresses the development of other nuclei, is proposed. This is obtained by decreasing gradually the temperature at the point of expected growth at a rate sufficiently low to equalize the protein concentration (under conditions where material is captured by growing crystal) via diffusive and convective processes. An individual growth of lysozyme single crystals in a capillary is demonstrated.
About the authors
V. V. Safronov
Space Materials Science Laboratory, Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Kaluga Branch
Author for correspondence.
Email: victor_safronov@yahoo.com
Russian Federation, Kaluga, 248640
N. V. Krivonogova
Space Materials Science Laboratory, Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Kaluga Branch
Email: victor_safronov@yahoo.com
Russian Federation, Kaluga, 248640
V. I. Strelov
Space Materials Science Laboratory, Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Kaluga Branch
Email: victor_safronov@yahoo.com
Russian Federation, Kaluga, 248640
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