Structural investigations of E. Coli dihydrolipoamide dehydrogenase in solution: Small-angle X-ray scattering and molecular docking
- Authors: Dadinova L.A.1,2, Rodina E.V.2, Vorobyeva N.N.2, Kurilova S.A.2, Nazarova T.I.2, Shtykova E.V.1,2
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Affiliations:
- Shubnikov Institute of Crystallography
- Moscow State University
- Issue: Vol 61, No 3 (2016)
- Pages: 414-420
- Section: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/189997
- DOI: https://doi.org/10.1134/S1063774516030093
- ID: 189997
Cite item
Abstract
Dihydrolipoamide dehydrogenase from Escherichia coli (LpD) is a bacterial enzyme that is involved in the central metabolism and shared in common between the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes. In the crystal structure, E. coli LpD is known to exist as a dimer. The present work is focused on analyzing the solution structure of LpD by small-angle X-ray scattering, molecular docking, and analytical ultracentrifugation. It was shown that in solution LpD exists as an equilibrium mixture of a dimer and a tetramer. The presence of oligomeric forms is determined by the multifunctionality of LpD in the cell, in particular, the required stoichiometry in the complexes.
About the authors
L. A. Dadinova
Shubnikov Institute of Crystallography; Moscow State University
Email: shtykova@ns.crys.ras.ru
Russian Federation, Leninskii pr. 59, Moscow, 119333; Moscow, 119992
E. V. Rodina
Moscow State University
Email: shtykova@ns.crys.ras.ru
Russian Federation, Moscow, 119992
N. N. Vorobyeva
Moscow State University
Email: shtykova@ns.crys.ras.ru
Russian Federation, Moscow, 119992
S. A. Kurilova
Moscow State University
Email: shtykova@ns.crys.ras.ru
Russian Federation, Moscow, 119992
T. I. Nazarova
Moscow State University
Email: shtykova@ns.crys.ras.ru
Russian Federation, Moscow, 119992
E. V. Shtykova
Shubnikov Institute of Crystallography; Moscow State University
Author for correspondence.
Email: shtykova@ns.crys.ras.ru
Russian Federation, Leninskii pr. 59, Moscow, 119333; Moscow, 119992