Small-angle scattering study of Aspergillus awamori glycoprotein glucoamylase
- Authors: Schmidt A.E.1, Shvetsov A.V.1,2, Kuklin A.I.3,4, Lebedev D.V.1, Surzhik M.A.1,2, Sergeev V.R.1,2, Isaev-Ivanov V.V.1
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Affiliations:
- Konstantinov Petersburg Nuclear Physics Institute
- St. Petersburg State Polytechnical University
- Joint Institute for Nuclear Research
- Research-Educational Centre “Bionanophysics”
- Issue: Vol 61, No 1 (2016)
- Pages: 149-152
- Section: Diffraction and Scattering of Neutrons
- URL: https://journals.rcsi.science/1063-7745/article/view/189755
- DOI: https://doi.org/10.1134/S1063774516010223
- ID: 189755
Cite item
Abstract
Glucoamylase from fungus Aspergillus awamori is glycoside hydrolase that catalyzes the hydrolysis of α-1,4- and α-1,6-glucosidic bonds in glucose polymers and oligomers. This glycoprotein consists of a catalytic domain and a starch-binding domain connected by an O-glycosylated polypeptide chain. The conformation of the linker, the relative arrangement of the domains, and the structure of the full-length enzyme are unknown. The structure of the recombinant glucoamylase GA1 was studied by molecular modelling and small-angle neutron scattering (SANS) methods. The experimental SANS data provide evidence that glucoamylase exists as a monomer in solution and contains a glycoside component, which makes a substantial contribution to the scattering. The model of full-length glucoamylase, which was calculated without taking into account the effect of glycosylation, is consistent with the experimental data and has a radius of gyration of 33.4 ± 0.6 Å.
About the authors
A. E. Schmidt
Konstantinov Petersburg Nuclear Physics Institute
Author for correspondence.
Email: schmidt@omrb.pnpi.spb.ru
Russian Federation, Orlova Roscha, Gatchina, Leningrad oblast, 188300
A. V. Shvetsov
Konstantinov Petersburg Nuclear Physics Institute; St. Petersburg State Polytechnical University
Email: schmidt@omrb.pnpi.spb.ru
Russian Federation, Orlova Roscha, Gatchina, Leningrad oblast, 188300; Politekhnicheskaya ul. 29, St. Petersburg, 195251
A. I. Kuklin
Joint Institute for Nuclear Research; Research-Educational Centre “Bionanophysics”
Email: schmidt@omrb.pnpi.spb.ru
Russian Federation, ul. Joliot-Curie 6, Dubna, Moscow region, 141980; Institutskii per. 9, Dolgoprudny, Moscow region, 141700
D. V. Lebedev
Konstantinov Petersburg Nuclear Physics Institute
Email: schmidt@omrb.pnpi.spb.ru
Russian Federation, Orlova Roscha, Gatchina, Leningrad oblast, 188300
M. A. Surzhik
Konstantinov Petersburg Nuclear Physics Institute; St. Petersburg State Polytechnical University
Email: schmidt@omrb.pnpi.spb.ru
Russian Federation, Orlova Roscha, Gatchina, Leningrad oblast, 188300; Politekhnicheskaya ul. 29, St. Petersburg, 195251
V. R. Sergeev
Konstantinov Petersburg Nuclear Physics Institute; St. Petersburg State Polytechnical University
Email: schmidt@omrb.pnpi.spb.ru
Russian Federation, Orlova Roscha, Gatchina, Leningrad oblast, 188300; Politekhnicheskaya ul. 29, St. Petersburg, 195251
V. V. Isaev-Ivanov
Konstantinov Petersburg Nuclear Physics Institute
Email: schmidt@omrb.pnpi.spb.ru
Russian Federation, Orlova Roscha, Gatchina, Leningrad oblast, 188300