Email this article Characterization of oligomerization of a peptide from the ebola virus glycoprotein by small-angle neutron scattering
- Authors: Egorov V.V.1,2, Gorshkov A.N.2, Murugova T.N.3, Vasin A.V.2,4, Lebedev D.V.1, Isaev-Ivanov V.V.1, Kiselev O.I.2
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Affiliations:
- Konstantinov Petersburg Nuclear Physics Institute
- Research Institute of Influenza
- Joint Institute for Nuclear Research
- Institute of Physics, Nanotechnology and Telecommunications
- Issue: Vol 61, No 1 (2016)
- Pages: 94-97
- Section: Diffraction and Scattering of Neutrons
- URL: https://journals.rcsi.science/1063-7745/article/view/189638
- DOI: https://doi.org/10.1134/S1063774516010065
- ID: 189638
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Abstract
Transmission electron microscopy (TEM) and small-angle neutron scattering (SANS) studies showed that model peptides QNALVCGLRQ (G33) and QNALVCGLRG (G31) corresponding to region 551–560 of the GP protein of the Sudan Ebola virus are prone to oligomerization in solution. Both peptides can form amyloid-like fibrills. The G33 peptide forms fibrils within one day of incubation, whereas the fibrillogenesis of the G31 peptide is observed only after incubation for several months. The possible role of the observed processes in the pathogenesis and the possibility of applying a combination of the TEM and SANS techniques to search for new compounds that are able to influence the protein oligomerization are discussed.
About the authors
V. V. Egorov
Konstantinov Petersburg Nuclear Physics Institute; Research Institute of Influenza
Author for correspondence.
Email: vlaegur@omrb.pnpi.spb.ru
Russian Federation, Orlova Roshcha, Gatchina, Leningrad oblast, 188300; ul. Professora Popova 15/17, St. Petersburg, 197376
A. N. Gorshkov
Research Institute of Influenza
Email: vlaegur@omrb.pnpi.spb.ru
Russian Federation, ul. Professora Popova 15/17, St. Petersburg, 197376
T. N. Murugova
Joint Institute for Nuclear Research
Email: vlaegur@omrb.pnpi.spb.ru
Russian Federation, ul. Joliot-Curie 6, Dubna, Moscow region, 141980
A. V. Vasin
Research Institute of Influenza; Institute of Physics, Nanotechnology and Telecommunications
Email: vlaegur@omrb.pnpi.spb.ru
Russian Federation, ul. Professora Popova 15/17, St. Petersburg, 197376; Politekhnicheskaya ul. 29, St. Petersburg, 195251
D. V. Lebedev
Konstantinov Petersburg Nuclear Physics Institute
Email: vlaegur@omrb.pnpi.spb.ru
Russian Federation, Orlova Roshcha, Gatchina, Leningrad oblast, 188300
V. V. Isaev-Ivanov
Konstantinov Petersburg Nuclear Physics Institute
Email: vlaegur@omrb.pnpi.spb.ru
Russian Federation, Orlova Roshcha, Gatchina, Leningrad oblast, 188300
O. I. Kiselev
Research Institute of Influenza
Email: vlaegur@omrb.pnpi.spb.ru
Russian Federation, ul. Professora Popova 15/17, St. Petersburg, 197376
