Pathological Mitosis and Mixoploidy in the Meristematic Tissues of Grape Plant

Abstract

The emergence of cells with pathological mitosis and the deviation from diploidity in the meristematic tissue of experimental plants of the genus Vitis (Tournef.) Linn. obtained from stenospermocarpic seeds was evaluated. The grape plants obtained from crossing seedless varieties with seedless and seed pollinators were the material for cytogenetic research. A total of 11 crosses were made. The cytogenetic analysis was carried out according to the recommended methods for fruit crops and grape. A total of 11 039 cells were examined. The pathology of mitosis in the cells of grape plants in vitro was observed, in particular, a hollow metaphase, a three-group metaphase and a metaphase with polar chromosomes as well as haploid, triploid, and tetraploid cells. The total frequency of abnormal cells was 0.15. The pathology of mitosis is observed in meristematic tissue of grape varieties as well. The evaluation of emergence of mitotic anomalies in the meristematic tissue of plants was carried out. The correspondence between the actual distribution of the anomalies in experimental populations and the theoretical distribution, as well as the degree of their relative habit to populations, the indices of absolute, relative, and intrapopulation diversity, was determined. As a result of the study of the polyembrionic hybrid offspring of grapes, differentiation in the equitability of distribution and habit of five mitotic anomalies to 11 populations was revealed, and differences between experimental populations by diversity were detected. The maximum degree of relative diversity of populations reached a value of 0.503. In general, the low relative diversity indicates a lack of complete equalization of anomalies by the presence in the populations.