Comparative ultrastructural analysis of the in vitro microspore embryoids and in vivo zygotic embryos of wheat as a basis for understanding of cytophysiological aspects of their development
- 作者: Seldimirova O.1, Kruglova N.1, Titova G.2,3, Batygina T.2,3
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隶属关系:
- Ufa Institute of Biology
- Komarov Botanical Institute
- St. Petersburg State University
- 期: 卷 48, 编号 3 (2017)
- 页面: 185-197
- 栏目: Developmental Biology of Plants
- URL: https://journals.rcsi.science/1062-3604/article/view/183587
- DOI: https://doi.org/10.1134/S1062360417030109
- ID: 183587
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详细
Ultrastructures of in vitro microspore embryoids and in vivo zygotic embryos of spring wheat have been analyzed and compared. Along with the similarity of ultrastructural characteristics of embryoid and embryo cells at the corresponding developmental stages, some differences have been revealed. Unlike embryos, embryoid cells are characterized by lipid inclusions and numerous mitochondria with well-developed internal membranes. According to our hypothesis, lipids represent an alternative energy source required for active cell divisions in the forming embryoids. Unlike embryos, since the earliest developmental stages, embryoid cells accumulate a significant amount of starch and then utilize it during the organogenesis and germination. A conclusion has been made that embryoid cells create their own reserve of carbohydrates, which is then mobilized during their development. The concept of T.B. Batygina (1987, 1997, 2014) about the universal character of the plant morphogenesis in vivo, in situ, and in vitro has been confirmed. The prospects for the use of microspore embryoidogenesis in vitro as a model to study cytophysiological aspects of zygotic embryogenesis in vivo are discussed.
作者简介
O. Seldimirova
Ufa Institute of Biology
编辑信件的主要联系方式.
Email: o_seldimirova@mail.ru
俄罗斯联邦, Ufa, 450054
N. Kruglova
Ufa Institute of Biology
Email: o_seldimirova@mail.ru
俄罗斯联邦, Ufa, 450054
G. Titova
Komarov Botanical Institute; St. Petersburg State University
Email: o_seldimirova@mail.ru
俄罗斯联邦, St. Petersburg, 197376; St. Petersburg, 199034
T. Batygina
Komarov Botanical Institute; St. Petersburg State University
Email: o_seldimirova@mail.ru
俄罗斯联邦, St. Petersburg, 197376; St. Petersburg, 199034