Morphological features of the transgenic tobacco plant shoot expressing the 3-hydroxy-3-methylglutagyl-CoA reductase (HMG1) gene in the direct and reverse orientations towards the promoter
- Authors: Ermoshin A.A.1, Kiseleva I.S.1, Bortsova S.A.1, Sanaeva Y.V.1, Alekseeva V.V.2
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Affiliations:
- Yeltsin Ural Federal University
- Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
- Issue: Vol 47, No 4 (2016)
- Pages: 216-222
- Section: Developmental Biology of Plants
- URL: https://journals.rcsi.science/1062-3604/article/view/183441
- DOI: https://doi.org/10.1134/S1062360416040044
- ID: 183441
Cite item
Abstract
3-Hydroxy-3-methylglutaryl-CoA reductase (HMG1) catalyzes the formation of mevalonic acid, the key intermediate of the cytosolic isoprenoid synthesis pathway. The parameters of stem and leaf growth were studied in the transgenic tobacco plants that express the HMG1 gene in both sense and antisense orientations towards the constitutive promoter. The transgenic plant height did not significantly differ from that of the control plants, though the plants carrying the sense copy of the HMG1 gene were considerably taller than plants that carried the antisense gene copy. Plants carrying an extra copy of the HMG1 gene were also characterized by increased leaf area. The number of mesophyll cells calculated per square unit of transgenic plants leaves was smaller than in the control plant leaves, though their volume was not considerably changed in any of the variants, suggesting changes in the cell packing density in leaves.
Keywords
About the authors
A. A. Ermoshin
Yeltsin Ural Federal University
Email: bos_o@mail.ru
Russian Federation, Ekaterinburg, 620000
I. S. Kiseleva
Yeltsin Ural Federal University
Email: bos_o@mail.ru
Russian Federation, Ekaterinburg, 620000
S. A. Bortsova
Yeltsin Ural Federal University
Author for correspondence.
Email: bos_o@mail.ru
Russian Federation, Ekaterinburg, 620000
Y. V. Sanaeva
Yeltsin Ural Federal University
Email: bos_o@mail.ru
Russian Federation, Ekaterinburg, 620000
V. V. Alekseeva
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
Email: bos_o@mail.ru
Russian Federation, Pushchino, 142290