Long-term sperm storage in the Siberian crane (Grus leucogeranus pallas): Analysis of paternity and relatedness under artificial insemination
- Authors: Mudrik E.A.1, Kashentseva T.A.2, Politov D.V.1
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Affiliations:
- Vavilov Institute of General Genetics
- Oka Crane Breeding Center
- Issue: Vol 47, No 3 (2016)
- Pages: 103-108
- Section: Developmental Biology of Vertebrates
- URL: https://journals.rcsi.science/1062-3604/article/view/183421
- DOI: https://doi.org/10.1134/S106236041603005X
- ID: 183421
Cite item
Abstract
Using ten microsatellite loci, paternity analysis has been conducted for 71 individuals of the Siberian crane (Grus leucogeranus Pallas) obtained under artificial insemination in Oka Crane Breeding Center in 2001–2014. The fathers of 39 chicks were the sires whose sperm was used for insemination directly before fertilized egg laying. Paternity of 23 fertilizations belonged to the sires whose sperm was used in the beginning or middle of insemination cycle. Nine cases of fertilization resulted from natural copulation of artificially inseminated females with their social partners. The terms of sperm storage in the female’s reproductive ducts before fertilization were 0–6 days in the case of paternity of the last sperm donor and 2–15 days in the case of competing sperm by previous donors. Genetic relatedness by microsatellite loci between breeders of the captive Siberian crane population does not prevent fertilization and does not always lead to inbreeding depression.
About the authors
E. A. Mudrik
Vavilov Institute of General Genetics
Author for correspondence.
Email: mudrik@vigg.ru
Russian Federation, ul. Gubkina 3, Moscow, 119991
T. A. Kashentseva
Oka Crane Breeding Center
Email: mudrik@vigg.ru
Russian Federation, Spasskii raion, pos., Brykin Bor, Ryazan oblast, 391072
D. V. Politov
Vavilov Institute of General Genetics
Email: mudrik@vigg.ru
Russian Federation, ul. Gubkina 3, Moscow, 119991