Differentiation of stem cells isolated from rat skeletal muscles towards cardiomyocytes: The effect of an inhibitor of DNA methylation 5-azacytidine
- Авторы: Balan O.1, Ozernyuk N.2
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Учреждения:
- Institute of Biology of Karelian Research Center
- Koltsov Institute of Development Biology
- Выпуск: Том 44, № 4 (2017)
- Страницы: 355-362
- Раздел: Cell Biology
- URL: https://journals.rcsi.science/1062-3590/article/view/182421
- DOI: https://doi.org/10.1134/S1062359017040021
- ID: 182421
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Аннотация
The effects of the inhibitor of DNA methylation 5-azacytidine on stem (satellite) cells isolated from fetal and definitive skeletal muscles of rats lead to the expression of marker genes of cardiomyogenesis (Gata4, Nkx2.5, connexin-43, n-cadherin, as well as Cacna 1 c encoding the cardiac subunit of the L-type Ca2+-channel). Through comparative analysis of the dynamics of expression of key markers of cardiomyogenesis, it was established that satellite cells isolated from fetal muscles have a more expressive potency to cardiomyocyte differentiation in vitro (expression of specific marker genes) compared to cells from muscles of adult animals. In the process of induced cardiomyocyte differentiation, the expression of MyoD and m-cadherin marker genes of skeletal muscle differentiation was not detected, suggesting that in the experiments presented the program of myogenic differentiation of skeletal muscles is inhibited.
Об авторах
O. Balan
Institute of Biology of Karelian Research Center
Email: ozernyuk@mail.ru
Россия, ul. Pushkinskaya 11, Petrozavodsk, Karelia, 185910
N. Ozernyuk
Koltsov Institute of Development Biology
Автор, ответственный за переписку.
Email: ozernyuk@mail.ru
Россия, ul. Vavilova 26, Moscow, 119991