Polymorphism and the secondary structure of the mitochondrial nad1 gene b/c intron in Malus species and related Rosaceae species


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Abstract

Nucleotide sequences polymorphism of mitochondrial nad1 gene b/c intron was studied in 41 Malus accessions and 21 related Rosaceae accessions. The b/c intron sequence in genus Malus appeared to be very conservative, while in other studied Rosaceae species 126 variable sites and indels were detected in the intron sequence that varied in length from 1124 to 1456 bp. The predicted b/c intron pre-mRNA secondary structure for Malus species was determined; IBS/EBS binding sites and the boundaries of the six functional domains were identified.

About the authors

M. A. Filyushin

Institute of Bioengineering, Research Center of Biotechnology

Author for correspondence.
Email: michel7753@mail.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071

K. V. Boris

Institute of Bioengineering, Research Center of Biotechnology; Vavilov Institute of General Genetics

Email: michel7753@mail.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071; ul. Gubkina 3, Moscow, 119991


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