Characterization of Phytase Transgenic Wheat under Salt Stress

Abstract

Wheat is the major cereal crop. It is grown to meet the demand of food in developing countries but salinity is the serious problem in some countries which are based on agriculture. In the present study fourteen transgenic lines expressing phytase gene were selected using PCR and BASTA leaf paint assay. Different morphological and biochemical analysis that include plant growth, number of spikes, length of spikes, chlorophyll, soluble protein, soluble sugar and proline content were carried out from the leaf of the plants under different stress level of salt. Results showed mostly significant reduction in all the morphological parameters and chlorophyll content with increase in salt concentration while on the other hand in all other parameters, most of them showed significant increment. The result showed that some lines have ability to resist salt stress.