Use of Dibutyryl Cyclic Adenosine Monophosphate in Sperm Capacitation for in vitro Production of Bovine Embryos
- Authors: Smetanina I.G.1, Tatarinova L.V.1, Krivokharchenko A.S.2
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Affiliations:
- All-Russia Research Institute of Animal Physiology, Biochemistry, and Nutrition, Ernst VIZh Federal Research Center
- Semenov Institute of Chemical Physics, Russian Academy of Sciences
- Issue: Vol 46, No 4 (2019)
- Pages: 327-331
- Section: Cell Biology
- URL: https://journals.rcsi.science/1062-3590/article/view/183062
- DOI: https://doi.org/10.1134/S1062359019040137
- ID: 183062
Cite item
Abstract
The opportunity to create a chemically defined system for bovine sperm in vitro capacitation has been performed. 100 µM dibutyryl cyclic adenosine monophosphate (dbcAMP) used as a capacitating agent instead of heparin 10 µg/mL solution was administered in a Tyrode’s protein-free medium for fertilization. In addition, the fertilization medium did not contain glucose or any sperm-activating additives. It has been proven for the first time that spermatozoa capacitated in the in vitro protein-free medium with the use of dbcAMP only are capable of highly efficient in vitro fertilization of the bovine oocytes, which were matured outside the body in a protein-free medium as well. Embryos developed to the 4- to 8-cell stage are chosen as criteria for normal fertilization.
About the authors
I. G. Smetanina
All-Russia Research Institute of Animal Physiology, Biochemistry, and Nutrition, Ernst VIZh Federal Research Center
Author for correspondence.
Email: sme.irina2011@yandex.ru
Russian Federation, Borovsk, Kaluga oblast, 240913
L. V. Tatarinova
All-Russia Research Institute of Animal Physiology, Biochemistry, and Nutrition, Ernst VIZh Federal Research Center
Email: sme.irina2011@yandex.ru
Russian Federation, Borovsk, Kaluga oblast, 240913
A. S. Krivokharchenko
Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: sme.irina2011@yandex.ru
Russian Federation, Moscow, 119991