Study of Dactylorhiza Seeds (D. baltica and D. maculata) from the Orchid Collection of the Cryobank at Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
- Authors: Nikishina T.V.1, Kozlova O.N.2, Levitskaya G.E.3, Vysotskaya O.N.1
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Affiliations:
- Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
- State Scientific Institution of Central Botanical Garden, National Academy of Sciences of Belarus
- Institute of Cell Biophysics, Russian Academy of Sciences
- Issue: Vol 46, No 3 (2019)
- Pages: 242-250
- Section: Botany
- URL: https://journals.rcsi.science/1062-3590/article/view/183045
- DOI: https://doi.org/10.1134/S1062359019030063
- ID: 183045
Cite item
Abstract
Seeds of two orchid species from the Dactylorhiza genus (D. baltica and D. maculata) collected in Belarus and in Russia and placed for long-term preservation into liquid nitrogen in the cryobank of the Timiryazev Institute of Plant Physiology were studied. The complex quality evaluation of collection specimens was carried out by using the seed viability rating and also by their morphometric and physiology characteristics. It was shown that comprehensive study of seed specimens collected from different habitats at different times may be synchronized by applying a cryopreservation technique and in vitro method for plant material cultivation.
About the authors
T. V. Nikishina
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
Email: cryo@ippras.ru
Russian Federation, Moscow, 127276
O. N. Kozlova
State Scientific Institution of Central Botanical Garden, National Academy of Sciences of Belarus
Email: cryo@ippras.ru
Belarus, Minsk, 220012
G. E. Levitskaya
Institute of Cell Biophysics, Russian Academy of Sciences
Email: cryo@ippras.ru
Russian Federation, Pushchino, Moscow oblast, 142290
O. N. Vysotskaya
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
Author for correspondence.
Email: cryo@ippras.ru
Russian Federation, Moscow, 127276