Molecular genetic and chemotaxonomic identification of the bacterium of the genus Ochrobactrum possessing oil-oxidizing and nitrogen-fixing activity
- Authors: Korshunova T.Y.1, Mukhamatdyarova S.R.1, Loginov O.N.1
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Affiliations:
- Ufa Institute of Biology
- Issue: Vol 44, No 5 (2017)
- Pages: 493-500
- Section: Microbiology
- URL: https://journals.rcsi.science/1062-3590/article/view/182459
- DOI: https://doi.org/10.1134/S1062359017050090
- ID: 182459
Cite item
Abstract
It was shown that analysis of cellular fatty acids using gas chromatography-mass-spectrometry and application of the gene encoding β-subunit of DNA gyrase (gyrB) as an alternative phylogenetic marker supplemented the taxonomic description of the biotechnologically valuable strain Ochrobactrum sp. IB DT-5.3/2 but were ineffective for species identification of the bacterium. However, based on the results of the matrixassisted laser desorption-ionization time of flight mass spectrometry of the protein fractions and our previous studies of the cultural–morphological and biochemical features, as well as sequencing of the 16S rRNA gene, we can conclude that the strain Ochrobactrum sp. IB DT-5.3/2 refers to the species O. intermedium.
About the authors
T. Yu. Korshunova
Ufa Institute of Biology
Author for correspondence.
Email: korshunovaty@mail.ru
Russian Federation, Ufa, 450054
S. R. Mukhamatdyarova
Ufa Institute of Biology
Email: korshunovaty@mail.ru
Russian Federation, Ufa, 450054
O. N. Loginov
Ufa Institute of Biology
Email: korshunovaty@mail.ru
Russian Federation, Ufa, 450054