Pine wilt disease and possible causes of its incidence in Russia
- Authors: Arbuzova E.N.1, Kulinich O.A.1,2, Mazurin E.S.3, Ryss A.Y.4, Kozyreva N.I.2, Zinovieva S.V.2
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Affiliations:
- All-Russia Center for Plant Quarantine
- Center for Parasitology, Severtsov Institute of Ecology and Evolution
- All-Russia Research Institute of Agricultural Biotechnology
- Zoological Institute
- Issue: Vol 43, No 4 (2016)
- Pages: 300-306
- Section: Microbiology
- URL: https://journals.rcsi.science/1062-3590/article/view/181594
- DOI: https://doi.org/10.1134/S1062359016040026
- ID: 181594
Cite item
Abstract
Surveys of forests and stockpiled timber of pine, spruce, larch, and silver fir in 14 administrative subjects of the Russian Federation revealed widespread occurrence of the coniferous wood parasitic nematode Bursaphelenchus mucronatus. Twenty species of bacteria belonging to 13 genera have been detected in 25 B. mucronatus isolates, and their identity has been determined by direct sequencing of the 16S RNA gene. The most frequently occurring were bacteria from the genera Pseudomonas, Stenotrophomonas, Pantoea, Bacillus, Burkholderia, and Serratia. Prevalence of Pseudomonas brenneri and P. fluorescence, which were also found in the nematode dauer larva (LIV) isolated from the fir sawyer beetle Monochamus urussovi, have also been assessed. Two nematode B. xylophilus isolates from Portugal and one isolate from the United States have been examined, and 10 symbiotic bacteria species have been isolated, including Agrobacterium tumefacience, P. fluorescens, P. brenneri, Rahnella aquatilis, Stenotrophomonas maltophilia, S. rhizophila, and Yersinia mollaretii.
About the authors
E. N. Arbuzova
All-Russia Center for Plant Quarantine
Email: okulinich@mail.ru
Russian Federation, ul. Pogranichnaya 32, Bykovo, Moscow region, 140150
O. A. Kulinich
All-Russia Center for Plant Quarantine; Center for Parasitology, Severtsov Institute of Ecology and Evolution
Author for correspondence.
Email: okulinich@mail.ru
Russian Federation, ul. Pogranichnaya 32, Bykovo, Moscow region, 140150; Leninskii pr. 33, Moscow, 119071
E. S. Mazurin
All-Russia Research Institute of Agricultural Biotechnology
Email: okulinich@mail.ru
Russian Federation, ul. Timiryazevskaya 42, Moscow, 127550
A. Yu. Ryss
Zoological Institute
Email: okulinich@mail.ru
Russian Federation, Universitetskaya nab. 1, St. Petersburg, 199034
N. I. Kozyreva
Center for Parasitology, Severtsov Institute of Ecology and Evolution
Email: okulinich@mail.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071
S. V. Zinovieva
Center for Parasitology, Severtsov Institute of Ecology and Evolution
Email: okulinich@mail.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071