Some characteristics of transgenic clones of mouse R1 line embryonic stem cells
- Authors: Drozd S.F.1, Surkov S.A.1, Glazkov M.V.1
-
Affiliations:
- Kol’tsov Institute of Developmental Biology
- Issue: Vol 43, No 4 (2016)
- Pages: 283-289
- Section: Genetics
- URL: https://journals.rcsi.science/1062-3590/article/view/181587
- DOI: https://doi.org/10.1134/S1062359016030031
- ID: 181587
Cite item
Abstract
Transgenic clones of mouse embryonic stem cells of the R1 line were received by transfection of plasmid linear vectors. The changes in the transgene structure during its integration into the genome of the target cells were investigated. Displacements were found on the flanks of the integrated transgene. It was found that multicopy tandem structures are formed in head–tail orientation at the transgene integration. It was noted that the number of copies of the integrated transgenes varies considerably, but the introduction of DNA fragments isolated from the nuclear envelopes into the flanks of the transgene normalizes the number of its copies.
About the authors
S. F. Drozd
Kol’tsov Institute of Developmental Biology
Author for correspondence.
Email: sdrozd@yandex.ru
Russian Federation, ul. Vavilova 26, Moscow, 119334
S. A. Surkov
Kol’tsov Institute of Developmental Biology
Email: sdrozd@yandex.ru
Russian Federation, ul. Vavilova 26, Moscow, 119334
M. V. Glazkov
Kol’tsov Institute of Developmental Biology
Email: sdrozd@yandex.ru
Russian Federation, ul. Vavilova 26, Moscow, 119334