IDENTIFICATION OF CELLS OBTAINED FROM BLOOD OF ONCOLOGICAL PATIENTS WITH HEMOCYTOFILTRATION


Cite item

Full Text

Abstract

This article presents the outcomes of our own research of the detection of circulating cells in the peripheral blood of 48 patients with oncological diseases, using hemocytofiltration. The observed circulating cells (21 cases, 43.8%) have been represented by three variants: more often in the form of “bare” atypical nuclei (76.2% cases), also single conserved tumor cells (14.3%) and large shapeless single cells, probably of non-epithelial nature (9.5%). The difficulties of identifying the obtained cells in light microscopy and technical aspects of the usage of immunocytochemical techniques have been discussed.

About the authors

Olga G. Grigoruk

Altai branch of N.N. Blokhin National Medical Research Center of Oncology; Altai Regional Oncology Dispensary; The Altai State Medical University

Email: cytolakod@rambler.ru
MD, Ph.D., DSci., Head of the Department of Clinical Laboratory Diagnostics (implementation of cytological research methods), Altai Regional Oncology Dispensary, Barnaul, 656043, Russian Federation Barnaul, 656049, Russian Federation; Barnaul, 656043, Russian Federation; Barnaul, 656038, Russian Federation

A. F Lazarev

Altai branch of N.N. Blokhin National Medical Research Center of Oncology; Altai Regional Oncology Dispensary; The Altai State Medical University

Barnaul, 656049, Russian Federation; Barnaul, 656043, Russian Federation; Barnaul, 656038, Russian Federation

A. A Chimitov

Buryat Regional Clinical Oncology Dispensary

Ulan-Ude, 670047, Russian Federation

T. D Khankhashanova

Buryat Regional Clinical Oncology Dispensary

Ulan-Ude, 670047, Russian Federation

L. M Bazulina

Altai Regional Oncology Dispensary

Barnaul, 656043, Russian Federation

Ya. N Shoykhet

The Altai State Medical University

Barnaul, 656038, Russian Federation

References

  1. Зубцов Д.А., Зубцова Ж.И., Лавров А.В., Легченко Е.В., Аладинский В.А., Потеряхина А.В., Гольдштейн Д.В. Циркулирующие опухолевые клетки (ЦОК) при раке молочной железы: прогностическая значимость и методы выделения. Труды МФТИ. 2012; 4(3): 18-26.
  2. Ковалев А.А., Грудинская Т.В., Кузнецова Т.П., Ковалев К.А. Гетерогенность циркулирующих опухолевых клеток. Онкология. 2012; 14(2): 126-9.
  3. Christiansen J.J, Rajasekaran A.K. Reassessing epithelial to mesenchymal transition as a prerequisite for carcinoma invasion and metastasis. Cancer Res. 2006; 66: 8319-26. doi: 10.1158/0008-5472.CAN-06-0410
  4. Thiery J.P., Sleeman J.P. Complex networks orchestrate epithelial-mesenchymal transitions. Nat. Rev. Mol. Cell Biol. 2006; 7(2): 131-42. doi: 10.1038/nrm1835
  5. Hayes D.F., Cristofanilli M., Budd G.T. et al. Circulating tumor cells at each follow-up time point during therapy of metastatic breast cancer patients predict progression-free and overall survival. Clin. Cancer Res. 2006; 12(14 Pt 1): 4218-24. doi: 10.1158/1078-0432.CCR-05-2821
  6. Gaforio J.J., Serrano M.J., Sanchez-Rovira P. et al. Detection of breast cancer cells in the peripheral blood is positively correlated with estrogen-receptor status and predicts for poor prognosis. Int. J. Cancer. 2003; 107(6): 984-90. doi: 10.1002/ijc.11479
  7. Giatromanolaki A., Koukourakis M.I., Kakolyris S. et al. Assessment of highly angiogenic and disseminated in the peripheral blood disease in breast cancer patients predicts for resistance to adjuvant chemotherapy and early relapse. Int. J. Cancer. 2004; 108(4): 62-7. doi: 10.1002/ijc.11593
  8. Serrano M.J., Sánchez-Rovira P., Delgado-Rodriguez M., Gaforio J.J. Detection of circulating tumor cells in the context of treatment: prognostic value in breast cancer. Cancer Biol. Ther. 2009; 8(8): 671-5.
  9. Бжадут О.Б., Гривцова А.Ю., Тупицын Н.Н., Тюляндин С.А. Циркулирующие опухолевые клетки в крови больных местно-распространённым и диссеминированным раком молочной железы. Вестник РОНЦ им. Н.Н. Блохина РАМН. 2007; 18(3): 19-22.
  10. Ненахова Ю.Н., Лядов В.К., Поддубная И.В. Оценка динамики циркулирующих опухолевых клеток в процессе неоадъювантной химиотерапии у больных с местно-распространённым раком молочной железы. Современная онкология. 2017; 19(3): 64-8.
  11. Григорьева В.Л. Циркулирующие опухолевые клетки как маркёры прогноза выживаемости больных раком лёгких. Сибирское медицинское обозрение. 2016; 5: 84-6.
  12. Непомнящая Е.М., Никипелова Е.А., Нистратова О.В., Новикова И.А., Водолажский Д.И., Ульянова Е.П. Параллели между циркулирующими опухолевыми клетками и некоторыми морфо-генетическими показателями при колоректальном раке. Журнал фундаментальной медицины и биологии. 2016; 2: 64-9.
  13. Riethdorf S., Soave A., Rink M. The current status and clinical value of circulating tumor cells and circulating cell-free tumor DNA in bladder cancer. Transl. Androl. Urol. 2017; 6(6): 1090-110. doi: 10.21037/tau.2017.09.16
  14. Li J., Fu W., Zhang W., Li P. High Number of Circulating Tumor Cells Predicts Poor Survival of Cutaneous Melanoma Patients in China. Med. Sci. Monit. 2018; 24: 324-31.
  15. Грех И.Ф., Яковлева М.П. Методы обнаружения опухолевых клеток в кровяном русле. Ленинград : Медицина. Ленингр. отд-ние; 1966.
  16. Pantel K., Woelfle U. Detection and molecular characterisation of disseminated tumour cells: implications for anticancer therapy. Biochim. Biophys. Acta. 2005; 1756(1): 53-64. doi: 10.1016/j.bbcan.2005.07.002
  17. Balic M., Dandachi N., Hofmann G., Samonigg H., Loibner H. et al. Comparison of two methods for enumerating circulating tumor cells in carcinoma patients. Cytometry B. Clin. Cytom. 2005; 68(1): 25-30. doi: 10.1002/cyto.b.20065
  18. Kaiser J. Cancer’s circulation problem. Science. 2010; 327(5969): 1072-4. doi: 10.1126/science.327.5969.1072
  19. Kowalewska M., Chechlinska M., Markowicz S., Kober P., Nowak R. The relevance of RT-PCR detection of disseminated tumour cells is hampered by the expression of markers regar-ded as tumour-specific in activated lymphocytes. Eur. J. Cancer. 2006; 42: 2671-4. doi: 10.1016/j.ejca.2006.05.036
  20. Ntouroupi T., Ashraf S., McGregor S. et al. Detection of circula-ting tumour cells in peripheral blood with an automated scanning fluorescence microscope. Br. J. Cancer. 2008; 99(5): 789-95. doi: 10.1038/sj.bjc.6604545
  21. Camara O., Jorke C., Hammer U. et al. Monitoring circulating epithelial tumour cells (CETC) to gauge therapy: in patients with disease progression after trastuzumab persisting CETC can be eliminated by combined lapatinib treatment. J. Cancer Res. Clin. Oncol. 2009; 135(4): 643-7. doi: 10.1007/s00432-008-0498-8
  22. Andreopoulou E., Yang L.-Y., Rangel K. et al. Comparison of assay methods for detection of circulating tumor cells in metastatic breast cancer: AdnaGen AdnaTest BreastCancer Select/DetectTM versus Veridex CellSearchTM system. Int. J. Cancer. 2011; 130(7): 1590-7. doi: 10.1002/ijc.26111
  23. Kagan M., Howard D., Bendele T. et al. A Sample Preparation and Analysis System for Identification of Circulating Tumor Cells. J. Clin. Ligand Assay. 2002; 25(1): 104-10.
  24. Van der Auwera I., Peeters D., Benoy I. et al. Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK19/mammaglobin RT-PCR in patients with metastatic breast cancer. Br. J. Cancer. 2010; 102(2): 276-84. doi: 10.1038/sj.bjc.6605472
  25. Tewes M., Aktas B., Welt A. et al. Molecular profiling and predictive value of circulating tumor cells in patients with metastatic breast cancer: an option for monitoring response to breast cancer related therapies. Breast Cancer Res. Treat. 2009; 115(3): 581-90. doi: 10.1007/s10549-008-0143-x
  26. Cristofanilli M. The biological information obtainable from circulating tumor cells. Breast. 2009; 18(3): 38-40. doi: 10.1016/S0960-9776(09)70270-X
  27. Tan S., Yobas L., Lee G., Ong C., Lim C. Microdevice for the isolation and enumeration of cancer cells from blood. Biomed. Microdevices. 2009; 11(4): 883-92. doi: 10.1007/s10544-009-9305-9
  28. Sun Y.-F., Yang X.-R., Zhou J., Qiu S.-J., Fan J., Xu Y. Circula-ting tumor cells: advances in detection methods, biological issues, and clinical relevance. J. Cancer Res. Clin. Oncol. 2011; 137(8): 1151-73. doi: 10.1007/s00432-011-0988-y
  29. Hayashi N., Yamauchi H. Role of circulating tumor cells and disseminated tumor cells in primary breast cancer. Breast Cancer. 2012; 19(2): 110-7. doi: 10.1007/s12282-011-0282-5
  30. Hofman V.J., Ilie M.I., Bonnetaud C. et al. Cytopathologic Detection of Circulating Tumor Cells Using the Isolation by Size of Epithelial Tumor Cell Method. Am. J. Clin. Pathol. 2011; 135(1): 146-56. doi: 10.1309/AJCP9X8OZBEIQVVI
  31. Paterlini-Bréchot P., Benali N.L. Circulating tumor cells (CTC) detection: clinical impact and future directions. Cancer Lett. 2007; 253(2): 180-204. doi: 10.1016/j.canlet.2006.12.014
  32. Weinberg R.A. Twisted epithelial-mesenchymal transition blocks senescence. Nat. Cell. Biol. 2008; 10: 1021-3. doi: 10.1038/ncb0908-1021

Copyright (c) 2018 Eco-Vector


 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies